氯霉素诱导白血病细胞系K562凋亡的实验研究

Study on the Apoptosis-inducing Effects of Chloramphenicol on K562 Leukemia Cells

[目的]观察氯霉素(CAP)对白血病细胞系K562的诱导凋亡作用。[方法]采用四唑盐(MTT)实验,集落培养实验观察CAP对K562细胞增殖的影响,采用Hoechest 33258荧光染色观察细胞形态学变化和流式细胞术(FCM)检测CAP作用后K562细胞凋亡峰的出现三方面评价CAP对K562细胞的诱导凋亡作用。[结果]CAP抑制K562细胞增殖,诱导细胞亚二倍峰的出现,并在形态上有诱导凋亡的改变。[结论]CAP有诱导K562细胞凋亡的作用。

[Objective]To determine the apoptosis-inducing effects of Chloramphenicol on K562 leukemia cells.[Methods]Apoptosis-inducing effects of a neurotransmitter Chloramphenicol were evaluated by proliferation,morphology and detection of apoptosis apex through flow cytometry.We evaluated the effects of Chloramphenicol on K562 cells proliferation by MTT assay,clony formation assay,and the morphology by Hoechest 33258 staining.[Results] Chloramphenicol inhibited proliferation of K562 cells,induced the apoptosis apex,and showed apoptosis morphology after drug treatment.[Conclusion] Chloramphenicol possessed apoptosis-inducing effects on K562 cells.