西洛他唑鼻腔给药对小鼠慢性脑缺血损伤的保护作用

Protective effect of intranasal cilostazol administration on chronic injury after cerebral ischemia in mice

目的:观察西洛他唑鼻腔给药对小鼠慢性脑缺血损伤的保护作用。方法:以大脑中动脉栓塞(MCAO)诱导小鼠局灶性脑缺血,分别于术后1、4、7 h给予西洛他唑(鼻腔给药或腹腔注射给药),从第2天起,每天2次,持续到缺血后14 d。缺血后1、3、7、14、21、28、35 d进行神经症状评分和斜板试验,并记录小鼠的生存率。实验结束后,测定脑损伤体积和神经元数量。结果:西洛他唑鼻腔给药0.3 mg/kg能显著提高小鼠MCAO术后35 d的生存率。西洛他唑鼻腔给药(0.3、1mg/kg)和西洛他唑腹腔给药(10 mg/kg)能改善缺血后神经症状评分,降低脑梗死体积,减轻脑萎缩,并不同程度地增加缺血侧不同脑区的神经元密度。结论:西洛他唑鼻腔给药对小鼠慢性缺血性脑损伤具有保护作用,可作为脑缺血治疗的一种新方法。

Objective:To investigate the protective effect of cilostazol administrated intranasally on chronic injury after focal cerebral ischemia in mice. Methods:Focal cerebral ischemia in mice was induced by middle cerebral artery occlusion(MCAO).Cilotazol was administrated intranasally or intraperitoneally 1 h,4 h and 7 h after the operation;then twice a day from the second day for 2 weeks.The neurological deficit scoring and the inclined board testing were performed within 35 d after ischemia.The survival rate,infarct volume and neuron density were assessed 35 d after ischemia. Results:Intranasal cilostazol at 0.3 mg/kg increased the survival rate.Intranasal cilostazol(0.3 mg/kg,1 mg/kg) and intraperitoneal cilostazol(10 mg/kg) significantly attenuated neurological deficit,reduced infarct volume,and increased the survival neuron density in the border of ischemia region. Conclusion:Cilostazol administered intranasally demonstrates protective effects on chronic cerebral ischemia in mice.