印尼姜黄与中国姜黄药材的质量标准对比研究

Comparative study of the quality standard between Rhizoma Curcuma Longae and Rhizoma Curcuma xanthorrhizae

目的:建立印尼姜黄药材的质量标准,并将其与中国药典收载的姜黄品种进行对比。方法:应用性状,显微鉴定等方法,以姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素为对照,采用TLC法进行鉴别以及高效液相色谱法同时对三种姜黄素进行含量测定。按照中国药典2010年版中方法对10批姜黄药材中的挥发油、水分、总灰分、酸不溶性成分、浸出物进行测定。结果:印尼姜黄药材中姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素平均含量分别不得低于1.3%、0.7%、0.06%,中国姜黄不得低于1.0%、0.25%、0.15%。印尼姜黄挥发油含量不得低于10.0%,水分不得过16.0%,总灰分不得过7.0%,酸不溶性灰分不得过1.0%,浸出物不得少于12.0%。结论:所建立的印尼姜黄和中国姜黄定性定量测定方法简单准确,能够为该药的质量控制提供有效数据。

Objective:To establish the method of quality control of Rhizoma Curcuma xanthorrhizae,and compare with the Rhizoma Curcuma Longae.Method:The study was carried out using characteristics and microscopic identification methods with curcumin,demethoxycurcumin and bidemethoxycurcumin as control.TLC was used as identification method and the contents were determined by HPLC.Determinations of the essential oil,moisture,ash content and acid insoluble elements in 10 batches of samples were carried out according to the appendix of Chinese Pharmacopoeia(2010 Edition).Result:The content of curcumin,demethoxycurcumin and bidemethoxycurcumin in Rhizoma Curcuma xanthorrhizae were not less than 1.3%,0.7%,0.06%,and in the Rhizoma Curcuma Longae were not less than1.0%,0.25%,0.15%.Rhizoma Curcuma xanthorrhizae had volatile oil not less than 10.0%,moisture not over 16.0%,total ash not over 7.0%,acid insoluble ash not over 1.0%,and extract not less than 12.0%.Conclusion:This method is simple,accurate with good reproducibility.Experimental results provide the basis for the identification,utilization,and development of quality standards of both Rhizoma Curcuma Longae and Rhizoma Curcuma xanthorrhizae.