中江石泉丹参丛枝菌根真菌鉴定

Identification of arbuscular mycorrhizal fungi of Salvia miltiorrhiza Bunge in Shiquan of Zhongjiang County

目的:研究丹参根系是否有丛枝菌根真菌的侵染、侵染情况、丛枝菌根真菌的种类,以便通过接种丛枝菌根真菌来提高丹参的产量和质量。方法:采用碱解离-酸性品红染色法对根样进行染色处理,观察侵染情况并计算侵染率;采用湿筛倾析-蔗糖离心法从土样中分离丛枝菌根真菌的孢子,利用形态学分类特征鉴定其种属。结果:丛枝菌根真菌能侵染丹参根系并形成典型的丛枝菌根,侵染率为17.6%;从丹参根际土样中共分离出13种丛枝菌根真菌,其中球囊霉属(Glomus)9种,无梗囊霉属(Acaulospora)3种,盾巨孢囊霉属(Scutell spora)1种,鉴定到种的有8种,分别是Glomus caledonium、Glomus clarum、Glomus constrictum、Glomus geosporum、Glomus mosseae、Glomus sinuosum、Acaulospora scobiculata、Acaulospora lacunosa。其中尚有5种只鉴定到属。结论:丛枝菌根真菌是丹参丰产栽培中的一种具有潜在应用价值的生物资源。

Objective:To investigate the colonization and component of arbuscular mycorrhizal fungi(AMF)association of Salvia miltiorrhiza Bunge in Shiquan of Zhongjiang County for the improvement of quality and quantity.Method:The arbuscular mycorrhizal colonization level was studied by alkaline lysis and acid fuchsin stain under light microscopy.And the spores of arbusular mycorrhizal fungi were isolated with wet sieve decantation-sugar centrifugation from field-collected root-rhizosphere and the genera were identified based on morphological characters.Result:Arbuscular mycorrhizal fungi can infect the root of Salvia miltiorrhiza Bunge and form typical arbuscular mycorrhiza with 17.6%colonization percentage.Specimens of spore or sporocarps of AMF were wet sieved from the collection of the rhizosphere soils,and 13 species of AMF were isolated from the soil sample.Among these 13 species,9 species belonged to Glomus,3 species belonged to Acaulospora,and only one belonged to Scutellospora.They were Glomus caledonium,Glomus clarum,Glomus constrictum,Glomus geosporum,Glomus mosseae,Glomus sinuosum,Acaulospora scobiculata and Acaulospora lacunose at species level.And another 5 were identified at genus level.Conclusion:Arbuscular mycorrhizal fungi may be a potent biology resource which can stimulate the growth of Salvia miltiorrhiza Bunge.