七氟醚对小鼠空间学习和记忆能力的影响

Effects of sevoflurane on spatial learning and memory in mice

目的通过Morris水迷宫实验,评价七氟醚麻醉对小鼠空间学习和记忆能力的影响。方法将24只C57BL/6品系小鼠分为麻醉组(n=12)和对照组(n=12),两组小鼠分别给予七氟醚麻醉120 min和吸纯氧120 min,均于次日开始进行Morris水迷宫实验,包括4 d训练和1 d探索实验。训练期记录平均游泳速度、潜伏期和游泳距离。探索期记录穿越平台次数、平台象限滞留时间和平台象限滞留时间百分比,并进行统计学分析。结果训练期第2～4天,麻醉组小鼠潜伏期明显短于对照组(P<0.05);在训练期第2天,麻醉组小鼠的游泳距离也明显短于对照组(P<0.05)。探索实验显示,与对照组比较,麻醉组小鼠平台象限滞留时间和平台象限滞留时间百分比明显增加(P<0.05)。结论七氟醚麻醉具有增强小鼠空间学习和记忆能力的作用。

Objective To observe the effects of sevoflurane anesthesia on spatial learning and memory in mice. MethodsTwenty-four C57BL/6 mice were divided into anesthesia group and control group(n=12).Since the following day,Morris water maze was performed on mice in both groups,including 4 trail days and 1 probe day.Latency,distance and average swimming velocity were recorded during trail days.Times across the platform,time during platform quadrant and time percent during platform quadrant were recorded on probe day.The data were statistically analyzed. Results From trail day 2-4,latencies of mice in anesthesia group were significantly shorter than those in control group(P<0.05).On trail day 2,distance of mice in anesthesia group was also significantly shorter than that of control group(P<0.05).On probe day,time during platform quadrant and time percent during platform quadrant in anesthesia group were significantly longer than those in control group(P<0.05). Conclusion Sevoflurane anesthesia improves not only learning ability but also spatial memory in mice.