含吡那地尔心脏保存液的供心保存效果

Effects of heart preservation solution containing Pinacidil on donor heart preservation

目的观察含不同浓度吡那地尔的心脏保存液对供心的保存效果。方法 40只SD大鼠根据供心保存方式随机分为正常对照组、Celsior液对照组、Celsior液+0.25 mmol/L吡那地尔组、Celsior液+0.5 mmol/L吡那地尔组和Celsior液+1 mmol/L吡那地尔组(n=8)。建立Langendorff离体心脏灌注模型,离体心脏置各保存液中6 h,再灌注30 min,观察并比较各组心功能指标[左心室收缩末压(LVESP)、左心室发展压(LVDP)、冠脉流量(CF)、左心室内压最大上升速率(dp/dtmax)和左心室内压最大下降速率(dp/dtmin)]、心肌超微结构和心肌细胞凋亡指数。结果与Celsior液对照组、Celsior液+0.25 mmol/L吡那地尔组和Celsior液+1 mmol/L吡那地尔组比较,Celsior液+0.5 mmol/L吡那地尔组大鼠供心LVESP、LVDP、CF、dp/dtmax和dp/dtmin显著提高(P<0.05),心肌细胞凋亡指数显著降低(P<0.05),且心肌细胞和线粒体结构较为完整。结论与单纯Celsior心脏保存液相比,含0.5 mmol/L吡那地尔的Celsior心脏保存液能减少供心保存后心肌细胞的凋亡,促进心功能的恢复,改善供心低温保存的效果。

Objective To observe the effects of heart preservation solution containing different concentrations of Pinacidil on donor heart preservation. Methods Forty SD rats were randomly divided into normal control group,Celsior solution control group,Celsior solution+0.25 mmol/L Pinacidil group,Celsior solution+0.5 mmol/L Pinacidil group and Celsior solution+1 mmol/L Pinacidil group according to different ways of donor heart preservation,with 8 rats in each group.Langendorff model of isolated heart was established,and isolated hearts were preserved in preservation solutions for 6 h,followed by reperfusion for 30 min.The parameters of cardiac function such as left ventricular end-systolic pressure(LVESP),left ventricular development pressure(LVDP),coronary flow(CF),maximum rate of left ventricular pressure rise(dp/dtmax) and minimum rate of left ventricular pressure rise(dp/dtmin),myocardial ultrastructure and myocardiocyte apoptotic index were observed and compared among groups. Results Compared with Celsior solution control group,Celsior solution+0.25 mmol/L Pinacidil group and Celsior solution+1 mmol/L Pinacidil group,the parameters of LVESP,LVDP,CF,dp/dtmax and dp/dtmin significantly increased(P0.05),the myocardiocyte apoptotic index significantly decreased(P0.05),and the structures of myocardiocytes and mitochondria were intact in Celsior solution+0.5 mmol/L Pinacidil group. Conclusion Compared with single Celsior heart preservation solution,Celsior heart preservation solution containing 0.5 mmol/L Pinacidil can decrease myocardiocyte apoptosis after donor heart preservation,promote recovery of cardiac function and improve the effects of hypothermic preservation of donor hearts.