牙周治疗对龈下菌斑中古细菌定植的影响

Effects of periodontal therapy on Archaea colonization in subgingival plaque

目的研究牙周治疗对龈下菌斑中古细菌定植的影响。方法对49例中重度牙周炎患者的牙周治疗进程进行跟踪,分别于龈上菌斑刮除后(基线)、牙周基础治疗后4周和牙周翻瓣术后12周,采集龈下菌斑标本抽提DNA,利用古细菌16S rRNA基因通用引物进行PCR,对各菌斑标本古细菌16S rRNA表达进行定性检测,确定阳性标本并计算古细菌检出率。采用Real-Tim e PCR技术对阳性标本中总细菌和古细菌16S rRNA基因进行定量检测,计算古细菌相对丰度。结果牙周翻瓣术后12周时龈下菌斑标本中的古细菌检出率和古细菌相对丰度分别为17.6%和0.58%,显著低于基线时的69.4%和2.32%及牙周基础治疗后4周时的57.1%和2.30%,差异均有统计学意义(P<0.05)。结论龈下菌斑中古细菌的定植情况伴随牙周治疗的进程而迅速降低,提示菌斑厌氧环境的改变是影响古细菌定植的关键因素。

Objective To investigate the effects of periodontal therapy on Archaea colonization in subgingival plaque.Methods The periodontal treatment process of 49 patients with moderate to severe periodontitis were followed up,and subgingival plaque samples were collected after supragingival plaque scaling(baseline),4 weeks after periodontal basic treatment and 12 weeks after periodontal flap surgery for DNA extraction.PCR was performed with 16S rRNA gene of Archaea,and the expression of 16S rRNA of Archaea in plaque samples was qualitatively detected.The positive samples were determined,and the detection rate of Archaea was calculated.Real-Time PCR was employed to quantitatively detect the 16S rRNA gene of total bacteria and Archaea in positive samples,and the relative abundance of Archaea was calculated.Results The detection rates of Archaea and relative abundance of Archaea in subgingival plaque samples 12 weeks after periodontal flap surgery were 17.6% and 0.58% respectively,which were significantly lower than those at baseline(69.4% and 2.32%) and 4 weeks after periodontal basic treatment(57.1% and 2.30%)(P<0.05). ConclusionThe Archaea colonization in subgingival plaque rapidly decreases with the periodontal treatment process,which indicates that the subgingival anaerobic environment is the key factor for Archaea colonization.