三种方法对大鼠视网膜固定效果的比较研究

Outcomes of rat retina fixation with three different methods

目的研究三种不同方法对大鼠视网膜石蜡切片的固定效果。方法根据离体眼球的不同固定方法,将9只SD大鼠随机分为A组(改良固定液固定,n=3)、B组(4%多聚甲醛固定,n=3)和C组(4%多聚甲醛心脏灌注法固定,n=3)。24 h后制作标本进行苏木精-伊红染色,分析比较固定效果。结果 A组固定的眼杯不变形,B组固定的有少许变形,C组固定的基本不变形。显微镜观察显示A组眼球视网膜各层组织结构完整,B组和C组视网膜均有断层、分离和脱片等现象。结论改良固定液固定眼球的方法优于单纯4%多聚甲醛和4%多聚甲醛心脏灌注固定的方法,适用于视网膜石蜡切片的后期研究。

Objective To investigate the outcomes of fixation of rat retina paraffin sections with three different methods.Methods Nine SD rats were randomly divided into group A(retina fixation with improved solution,n=3),group B(retina fixation with 4% paraformaldehyde,n=3) and group C(retina fixation with cardiac perfusion of 4% paraformaldehyde,n=3).Paraffin sections were prepared 24 h after retina fixation,HE staining was performed,and the outcomes of fixation were compared. Results There was no eyecup deformation in group A,a few eyecup deformations in group B and few eyecup deformation in group C.It was observed by microscopy that each layer of the retina structure was intact in group A,and there was retinal detachment in group B and group C. Conclusion Eyeball fixation with improved fixation solution may result in better outcomes than fixation with 4% paraformaldehyde or fixation with cardiac perfusion,and is suitable for further research with retinal paraffin sections.