β内酰胺抗生素头孢曲松对创伤性脑损伤大鼠海马谷氨酸盐水平的影响

Effects of β-lactam antibiotics ceftriaxone on expression of glutamate in hippocampus after traumatic brain injury in rats

目的:探讨β内酰胺抗生素头孢曲松对创伤性脑损伤大鼠海马谷氨酸盐水平的影响。方法:采用大鼠自由落体打击伤模型,随机分成3组:假手术对照组,创伤组,创伤+头孢曲松组。创伤+头孢曲松组致伤后立即腹腔注射头孢曲松。伤后3、12和24 h分别测定脑组织含水量,高效液相色谱法测定大鼠伤后12 h海马谷氨酸盐水平,在光镜下观察伤后24 h大鼠病理形态学变化。结果:与创伤组比较,创伤+头孢曲松组各时间点脑组织含水量明显减轻(P<0.05),海马CAl区神经元死亡减少,伤后12 h大鼠海马谷氨酸和天冬氨酸水平显著下降(P<0.05)。结论:β-内酰胺抗生素头孢曲松可以降低创伤性脑损伤大鼠海马谷氨酸盐水平,减轻脑水肿和神经元死亡。

Objective: To investigate the effect of β-lactam antibiotics ceftriaxone on the levels of glutamate in hippocampus following traumatic brain injury in rat. Methods: Rats were divided randomly into three groups:sham group;trauma group and trauma+ceftriaxone group.Rats in the later two groups were subjected to a lateral cortical impact injury caused by a free-falling object,and trauma+ceftriaxone group was treated with ceftriaxone immediately after injury.The levels of glutamate in hippocampus was determined by HPLC at 12 h after trauma;the pathological change at 24 h after trauma was examined and water content of brain tissue at 3,12 and 24 h after trauma was measured in three groups. Results: Compared with the trauma group,the water content in brain tissue(P<0.05),neuronal death in hippocampus CAl area and the levels of glutamate and aspartate in hippocampus at 12 h(P<0.05) were significantly decreased in trauma+ceftriaxone group. Conclusion: β-lactam antibiotics ceftriaxone can reduce the level of brain glutamate,brain edema and neuronal death after traumatic brain injury in rat.