LaCl_3和CPZ对磷饥饿番茄液泡膜H^+-ATPase和H^+-PPase活性的影响

Effects of LaCl_3 and CPZ on Tonoplast H^+-ATPase and H^+-PPase Activities in Tomato Seedlings During Phosphate Ptarvation

采用水培法研究了LaCl3和氯丙嗪(CPZ)对磷饥饿番茄幼苗液泡膜H+-ATPase和H+-PPase活性的影响。结果表明:LaCl3处理抑制了液泡膜H+-ATPase活性,但提高了H+-PPase活性;而CPZ处理既抑制了液泡膜H+-ATPase活性,又抑制了液泡膜H+-PPase活性。同时,CPZ处理引起磷饥饿番茄幼苗可溶性蛋白质含量下降,LaCl3处理引起可溶性蛋白质含量增加。

The effect of calmodulin antagonist chlorpromazine(CPZ) and an inhibitor of Ca2+ channel in plasma membrane La3+ on the activities of tonoplast H+-ATPase and H+-PPase of tomato seedlings under phosphate starvation were studied.The results showed that under phosphate starvation,the activities of tonoplast H+-ATPase and H+-PPase were increased in the roots of tomato seedlings.But this enhancement was abolished by CPZ.Compared with CPZ,treatment with La3+ decreased the activity of H+-ATPase and enhanced H+-PPase activity.Furthermore,CPZ caused the content of soluble proteins decreased but LaCl3 caused soluble increased in the seedlings during phosphate starvation.