摘要
对采自山东省不同葡萄园类别的葡萄样品,采用PTA-ELISA法和Dot-ELISA法对葡萄茎痘相关病毒(GRSPaV)进行了检测,采用DAS-ELISA法对葡萄卷叶病的3个毒原(GL-RaV-1-、2-、3)进行了检测。GRSPaV检测结果表明:检测的61个品种518个样品,阳性样品率33.6%,阳性品种率73.8%,其中资源圃样品阳性率32.7%,生产园样品阳性率34.0%。对检测到阳性样品辅以RT-PCR法验证,2对引物均扩增出了预期片段,分别为解旋酶基因340 bp片段、CP基因842 bp片段。GLRaV-1-、2-、3的检测结果表明:鲜食品种3种病原的阳性样品率分别为33.7%、7.1%、62.2%,酿酒品种阳性样品率分别为35.1%、16.2%、54.1%,综合3种病毒的阳性样品率为76.3%,品种感染率87.7%。通过该类研究对推动国内葡萄无毒化生产有重要意义。
The study had detected grapevine rupestris stem pitting associated virus(GRSPaV) of grapevine samples collected from different vineyards category in Shandong province,with PAT-ELISA and Dot-ELISA.Meanwhile,3 types of toxogenes of grapevine leafroll associated virus-1,-2,-3(GLRaV-1,-2,-3) had also been detected with DAS-ELISA.The results of GRSPaV detection were,among 518 samples of 61 cultivars,33.6% sample positive rate,73.8% cultivar positive rate;the sample positive rate of grapevine germplasm,production garden accounted for 32.7%,34.0%,respectively.With 2 pairs of designed primers,the samples showing positive reaction in ELISA tests were complemented by RT-PCR,anticipated fragments,340 bp for the helicase gene,842 bp for the CP gene having been amplified successfully.The results of GLRaV-1,-2,-3 detection of table grape had sample positive rates of 33.7%,7.1%,62.2%,respectively,those of wine grape 35.1%,16.2%,54.1%,respectively;the comprehensive positive samples,cultivars rate of the 3 kinks of viruses were,76.3%,87.7%,respectively.By the study,there will be of far reaching importance in propelling domestic grapevine virus-free production.
出处
《北方园艺》
CAS
北大核心
2011年第7期20-23,共4页
Northern Horticulture
基金
山东省自然科学基金资助项目(Y2006D32)
