摘要
以南岭莪术根茎上的芽为外植体,采用不同激素浓度的培养基对其进行了芽诱导、丛生芽继代、试管苗生根等研究。结果表明:在MS培养基中添加TDZ 0.05mg/L时,不定芽的诱导率最高,达到90%;丛生芽的增殖培养以MS+TDZ 0.3mg/L培养基为最佳,增殖倍数达到15.8,同时在芽的基部自发形成不定根;将长约3～4cm的生根苗切出,转入1/2MS基本培养基上壮苗,约4周后即可出瓶移栽,成活率达98%以上。
An in vitro protocol for plantlet regeneration of Curcuma kwangsiensis var nanlingensis had been optimized. Adventitious shoots were induced by culture of bud explants for 2 weeks in MS medium supplemented with 0.05 mg/L TDZ.The regeneration rate was up to 15.8 shoots/explant on MS medium with 0.3 mg/L TDZ.Rooting was spontaneous and the regenerated plants were transplanted in 1/2 MS basal medium for further development.Then the in vitro induced plants were successfully transferred to sand with 98%survival rate.
出处
《北方园艺》
CAS
北大核心
2011年第8期151-153,共3页
Northern Horticulture
基金
广东省科技攻关资助项目(2008B020400001)
中科院百人才计划资助项目
关键词
南岭莪术
组织培养
芽
生根
Curcuma kwangsiensis var.nanlingensis
tissue culture
axillary bud
rooting
