摘要
以紫花三角梅和紫红重瓣三角梅为外植体,进行丛生芽增殖比较研究,以期筛选出丛生芽诱导和增殖的最佳培养基。结果表明:愈伤组织丛生芽增殖最佳培养基为MS+BA2.0mg/L(2.5mg/L)+IBA 0.2mg/L+GA_31.0mg/L,增殖系数达7.7以上;侧芽途径丛生芽的增殖最佳培养基为MS+BA 3.0mg/L+IBA 0.1mg/L,增殖系数达4.0以上。考虑生产成本和生产周期,侧芽途径优于愈伤组织途径。
This paper maily dealt with the comparative research on multiplication of shoots through the organ culture with cv.Paper flower and B.spectabilis Willd as explant,in the hope of finding out the best explants medium culture procedure of shoots regeneration and multiplication.The results showed that the multiplication of callus induced shoot clumps:MS + BA2.5 mg/L+IBA 0.2 mg/L+GA_3 1.0 mg/L was the best medium.The multiplication rate of shoot clumps could be up to 7.7.The multiplication of side buds:MS+BA 3.0 mg/L+IBA 0.1 mg/L was the best medium,the multiplication rate of side buds coud be up to 4.0.As a result of the comparing of the two shoot clumps multiplication,the method of side buds was superior.
出处
《北方园艺》
CAS
北大核心
2011年第8期164-166,共3页
Northern Horticulture
关键词
三角梅
植株再生途径
丛生芽增殖
Bougainvillea glabra
plant regeneration methoeds
multiplication of shoot
