五种枸杞植物胚乳培养三倍体的诱导研究

Five Triploid Plants of Lycium barbarum L. Induced from Endosperm-cultural

选取宁夏农林科学院枸杞研究中心培育的5种优质枸杞品种(系),研究了不同激素配比的培养基以及胚因子对不同基因枸杞胚乳诱导及分化的影响,以选择适合不同品种枸杞胚乳植株再生的最佳条件,从而进行三倍体育种。结果表明:5种材料都诱导出了愈伤组织,在不去胚情况下,最高诱导效率达到80.0%;将愈伤组织分别转入5种激素配比的分化培养基中,MS+0.2 mg/L 6-BA+0.01 mg/L NAA+3%蔗糖+0.4%琼脂为最适分化培养基,且宁杞3号分化率最高达10.7%;将分化出的绿色小芽转入1/2MS+0.01 mg/L 6-BA+0.1 mg/L NAA生根培养基中诱导生根,15 d后形成再生植株。

Take five genotypes of Lycium barbarum L.as material,the endosperm which removal and not removal the embryo on 6 kinds MS medium with different hormone content.The results showed that the callus was successfully induced from the endosperm of five genotypes.With the embryo,the highest induction ratio of the endosperm callus was 80.0%.The callus were then transferred to 5 kinds differentiation medium with different hormone content.The best medium was MS+0.2 mg/L 6-BA+0.01 mg/L NAA+3% Sucrose+0.4% Agar,and the differentiation ratio of Ningqi No.3 was the highest reached 10.7% After culturing the buds on rooting medium(1/2MS+0.01 mg/L 6-BA+0.1 mg/L NAA)for 15 d,complete plants were obtained.