摘要
以从河北邯郸女贞上分离的锈菌为研究材料,经显微观测,锈菌为黄褐色,形状为卵圆形、梨形或近球形,大小为(15~21.5)μm×(16.5~22)μm。利用微波法快速提取了女贞锈病DNA,并利用保守引物扩增核糖体28 Sr DNA异变区,经聚类分析表明:女贞锈菌为柄锈菌科,与该科的白蜡柄锈菌及酸浆柄锈菌亲缘关系最近。该试验首次对女贞锈菌从分子水平进行分类及检测,对女贞锈病的防治具有重要意义。
Privet leaf samples with symptoms of a rust disease were collected from handan city in Hebei province and morphological characteristics of spore were identified based on microscopic examination.Urediniospores were tawny,globose to pear shaped and measured(15～21.5)μm×(16.5～22) μm.Rust DNA were extracted with microwave and the D1/D2/D3 domain of 28S rDNA were amplified by PCR using the conservative primer.The Phylogenic analysis revealed privet rust belong to Pucciniaceae and was most closely related to Puccinia sparganioidis.This is the first report of detection of privet rust based on PCR amplification which would provide basic information for further drawing up control measure.
出处
《北方园艺》
CAS
北大核心
2011年第9期174-176,共3页
Northern Horticulture
基金
河北邯郸市社科联基金资助项目(201053)
关键词
女贞
锈病
PCR扩增
聚类分析
Glossy privet
rust disease
PCR amplification
phylogenic analysis
