摘要
以百子莲花蕾为外植体,成功建立了其离体再生体系。结果表明:百子莲花蕾在诱导培养基MS+6-BA 4mg/L+NAA 0.1mg/L可分化出不定芽;在MS+6-BA 2mg/L+NAA 0.2mg/L+GA_3 0.1mg/L培养基上,百子莲不定芽增殖倍数可达到5.07;百子莲不定芽适宜的生根培养基为1/2MS+NAA 0.1mg/L+AC 0.1g/L,生根率可达100%,且百子莲组培苗移栽1个月后成活率可达到95%以上。
An in vitro plant regeneration system of Agapanthus praecox ssp.Orientalis 'Big Blue' was established using buds as explants.The results showed that the suitable adventitious buds inducing medium was murashige and skoog (MS)+NAA 0.1 mg/L+6-BA 4.0 mg/L.Adventitious buds were effectively generated and proliferated on MS+ 6-BA 2.0 mg/L+NAA 0.2 mg/L+GA_3 0.1 mg/L,which the multiple for multiplication was up to 5.08.The best rooting medium was 1/2MS+NAA 0.1 mg/L+AC 0.1 g/L with a rooting rate 100%.The plantlet was transplanted with a transplanting survival rates up to 95%after a month.
出处
《北方园艺》
CAS
北大核心
2011年第10期118-120,共3页
Northern Horticulture
关键词
百子莲
花蕾
不定芽
植株再生
Agapanthus praecox ssp.Orientalis 'Big Blue'
buds
adventitious buds
regeneration system
