山葡萄种质资源原花青素分布及其含量动态变化

The Distribution and Dynamic Changes of Procyanidin Contents in Vitis amurensis Germplasm Resources

采用香草醛-HPLC结合法对39份山葡萄种质资源及"贝达"的种子、果皮中原花青素含量进行测定,比较了山葡萄种质资源间原花青素含量和分布的特点与差异,并对其中4个山葡萄品种"双优"、"双红"、"双丰"和"双庆"的叶片、茎皮、果皮和种子原花青素动态含量进行测定,分析了原花青素在山葡萄各器官中的变化规律。结果表明:山葡萄种质资源原花青素含量存在较大变异,成熟果实种子与果皮原花青素含量变化幅度较大,变化范围分别为0.42～8.00mg/gFW和1.2～14.7mg/g FW;野生资源与选育品种间、不同花性种质间、不同地域种质间原花青素含量差异均不显著;果皮中原花青素含量高于种子,且差异显著;4个山葡萄品种不同器官原花青素含量动态变化与欧亚种酿酒葡萄存在明显差异,叶片原花青素含量在青果期升高,转色期下降,成熟期小幅度升高;茎皮原花青素含量在青果期升高,转色期、成熟期下降;果皮中原花青素含量在青果期,转色期缓慢升高,成熟期迅速升高;种子原花青素含量在青果期、转色期升高,成熟期下降。

The contents of procyanidin were detected by Folin-Ciocalteu method in combination with HPLC.The characteristics of distribution and contents of procyanidin in 39 Vitis amurensis germplasm resources and 'Beta' were determined to reveal the differences and characteristics among different germplasm resources.And dynamic changes of procyanidin contents in leaves,stem bark,peel and seed of 4 Vitis amurensis varieties 'Shuangyou','Shuanghong', 'Siangfeng','Shuangqing' were studied to reveal the law of changes of the procyanidin contents in different organs.The results showed that the contents of procyanidin in seeds and peel varied greatly,the value ranged from 0.42～8 mg/g FW and 1.2～14.7 mg/g FW,respectively.The difference of contents of procyanidin was not significant among different flowers sex germplasm,among different regions germplasm.The contents of anthocyanidins in Peel were significantly greater than seeds.The dynamic changes characteristics of Procyanidin contents in different organs of 4 main cultivars: The contents of anthocyanins in leaves increased in young fruiting period,and declined in turning-color period,and increased slightly in maturing stage;The contents of anthocyanins in stem bark increased,and declined in turning-color period and maturing stage;The contents of anthocyanins in peel increased slowly in young fruiting period and turning-color period,and increased rapidly in maturing stage;It increased in young fruiting period and hading period,and declined in maturing stage.