槲皮素对大鼠肾上腺嗜铬细胞瘤PC12细胞的诱导分化作用

Differentiation-inducing effects of quercetin on rat adrenal pheochromocytoma PC12 cells

目的探讨槲皮素对大鼠肾上腺嗜铬细胞瘤PC12细胞的诱导分化作用。方法 MTT法检测神经生长因子(NGF)、不同浓度槲皮素(5、12.5、25、50μmol/L)对PC12细胞增殖活性的影响。分别以NGF、不同浓度的槲皮素(5、12.5、25μmol/L)诱导PC12细胞分化,以PBS作为对照组,诱导3 d后,显微镜下观察并记录PC12细胞分化情况,统计分析细胞分化率(轴索长度≥细胞直径的细胞百分数)、具有轴索的细胞数、细胞平均轴索数、梭形细胞比例等细胞分化的形态学参数。结果 MTT检测发现:50μmol/L槲皮素可明显抑制PC12细胞增殖(P<0.05)。与对照组比较,NGF组、槲皮素组(5、12.5、25μmol/L)的细胞均表现出显著分化特征(均P<0.05)。不同浓度槲皮素组(5、12.5、25μmol/L)诱导PC12细胞分化能力没有明显统计学差异。结论槲皮素可诱导PC12细胞分化,但没有明显剂量依赖性。

Objective To explore the differentiation-inducing effect of quercetin on rat adrenal pheochromocytoma PC12 cells.Methods Proliferation of PC12 cells was evaluated by MTT [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide] assay after treatment with nerve growth factor(NGF) and different concentrations of quercetin(5,12.5,25,50μmol/L) which were administrated to induce differentiation of PC12 cell at the same time,and the cells treated with PBS served as the control group.The differentiation of PC12 cells was observed under light microscope and recorded three days after treatment.The morphology parameters such as cell differentiation rate(the percent of cells whose neuraxial length was equal or longer than its diameter),the number of cells with neuritis,average neuraxial number of per cell and percentage of fusiform cells were analyzed statistically.Results MTT assay showed that 50 μmol/L quercetin inhibited proliferation of PC12 cells significantly(P<0.05).Compared with the control group,the cells in NGF and different concentrations of quercetin(5,12.5,25μmol/L) groups showed significant differentiation characteristics(all P<0.05).There was no significant difference in capacity of inducing differentiation of PC12 cells between different concentrations of quercetin(5,12.5,25μmol/L).Conclusions The quercetin can induce the differentiation of PC12 cells,but has no significant dose-independence.