急性髓系白血病患者SFRP2基因甲基化的检测及临床意义

Detection and clinical significance of SFRP2 promoter methylation status in patients with acute myeloid leukemia

目的检测分泌型卷曲相关蛋白2(SFRP2)基因在急性髓系白血病(AML)患者中的甲基化状态,探讨SFRP2基因启动子区甲基化状态与AML发生的关系。方法收集了99例AML患者的骨髓或外周血样本,匹配以70例门诊普通患者的外周血作为对照。采用甲基化特异性PCR(MSP)方法对所有研究样本进行SFRP2基因启动子区甲基化状况检测。结果 99例AML患者中有27例检测到SFRP2基因的甲基化,甲基化率为27.3%;而正常对照组中未检出SFRP2基因的甲基化;两者比较差异有统计学意义(P<0.001)。SFRP2基因的甲基化状态与AML的年龄、性别、临床分型及样本来源(骨髓/外周血)间均未见显著关系(P>0.05)。结论 SFRP2基因启动子区的甲基化与AML的发生有相关性,可能是引起AML的分子机制之一。外周血中的SFRP2基因的甲基化可望作为一项新的分子标记物应用于AML的临床早期检测。

Objective To detect secreted frizzled related protein 2 (SFRP2) gene promoter methylation status in patients with acute myeloid leukemia (AML),and to explore whether the methylation status of SFRP2 gene is associated with the occurrence of AML.Methods 99 bone marrow or peripheral blood samples of AML patients was collected,and out-matched with 70 normal peripheral blood samples from volunteers of general outpatients.Methylation specific PCR (MSP) method was used to detect the promoter methylation status of SFRP2 gene for all samples.Results The abnormal methylation of SFRP2 gene was detected in 27 samples (27.3%) of AML patients;while none was detected in controls (P<0.001).There was no significant associations between the methylation status of SFRP2 gene and age,gender,the clinical classification or the sample source (bone marrow/blood) of AML patients (P>0.05).Conclusions The promoter methylation of SFRP2 gene plays an important role in the occurrence of AML.Promoter methylation status of SFRP2 gene in peripheral blood may serve as an useful biomarker for early diagnosis of AML.