凋亡抑制蛋白Livin在大肠腺瘤-癌序列中的表达差异及意义

Expression of Livin mRNA in colorectal carcinoma,adenoma,and normal colorectal mucosa and its clinical significance

目的探讨凋亡抑制蛋白Livin在大肠癌、大肠腺瘤及正常大肠黏膜中的表达差异及在不同临床病理特征的大肠癌中的表达差异。方法应用免疫组化ABC法检测Livin在正常大肠黏膜、大肠腺瘤及大肠癌中的表达部位;应用实时荧光定量聚合酶链反应法检测LivinmRNA在64例大肠癌、36例大肠腺瘤及12例正常黏膜中表达量的差异及其意义。结果 (1)Livin在正常大肠黏膜中不表达,在大肠腺瘤组织中,Livin少量表达在腺泡的底部,而在大肠腺癌组织中呈全层上皮高表达,但两者在LivinmRNA水平上的表达量差异有统计学意义(P=0.001)。大肠腺瘤与正常黏膜相比较,LivinmRNA表达上调,但差异无统计学意义(P>0.05)。(2)在大肠癌组织中,LivinmRNA在直肠癌组中表达高于结肠癌组(P=0.001),LivinmRNA表达与患者的性别、年龄、肿瘤分化程度、Duke′s分期、淋巴转移及肿瘤组织类型无关,与肿瘤浸润深度有相关性。结论 (1)凋亡抑制蛋白Livin参与了大肠肿瘤的发生,且在大肠腺瘤-腺癌阶段起到了作用。(2)Livin基因在直肠癌中表达更高,其高表达与大肠癌的浸润密切相关。

Objective To investigate the expression differences of Livin mRNA and protein among colorectal adenocarcinoma,colorectal adenoma and normal mucosa,and among different clinicopathological types of adenocarcinomas.Methods 112 specimens,including 36 colorectal adenomas,64 colorectal adenocarcinoma,and 12 normal mucosa were included.Each specimen was divided into two parts,one for immunohistochemistry and the other for real-time RT-PCR.Expression differences of Livin mRNA among colorectal adenocarcinoma,adenoma and normal colonic mucosa were evaluated by REST-XL software.Results The expression of Livin was examined in the basal third to half of the colorectal crypts in adenomas,while throughout the epithelium in the colorectal adenocarcinomas,and not examined in the normal mucosa.However,the expression of Livin mRNA in the adenocarcinomas was significantly higher than that in the adenomas(P=0.001).The significant difference of Livin mRNA expression between adenomas and normal mucosa were not obtained by REST-XL software(P>0.05).The expression of Livin mRNA in rectum cancer was significantly higher than that in colonic cancer(P=0.001),Livin mRNA expression in cancer's invasive depth at serosa or penetrate the serosa was significantly elevated than those cancer which encroach into submucosa or proper muscle(P=0.009),and Livin mRNA expression was no correlation with patient's age,sex,and tumor's type,degree of cell differentiation,lymph nodes metastasis,pathologic category,Duke's stage.Conclusions The difference in expression of Livin between adenoma and adenocarcinoma indicated the potential value of it in carcinogenesis of colorectal cancer.