MKN-45人胃癌实体瘤的荷瘤裸鼠原位移植肿瘤组织和癌旁远近端组织肿瘤相关因子基因蛋白表达的实验研究

Experimental study on factor of tumor-related gene expression in tumor tissue,adjacent proximal and distal of MKN-45 human gastric cancer orthotopic transplantation

目的胃癌的手术等治疗方法将肿瘤切除后仍易复发转移,检测肿瘤组织及癌旁远近端组织中肿瘤相关因子表达,明确改善残胃或机体的肿瘤易发微环境的重要性。方法裸鼠原位移植后,取MKN-45胃癌组织、癌旁远近端组织,免疫组化MaxVision二步法检测血管内皮生长因子(VEGF)、激酶功能区受体(KDR)、E-钙黏素(E-Cad)、环氧合酶-2(COX-2)、端粒酶、细胞间黏附分子-1(ICAM-1)基因蛋白表达。结果肿瘤组织与肿瘤近端、远端VEGF、KDR、E-Cad、COX-2、ICAM-1、端粒酶的表达差异均有统计学意义(P<0.01),肿瘤近端与肿瘤远端组织比较VEGF、KDR表达差异有统计学意义(P<0.01),E-Cad、端粒酶表达差异有统计学意义(P<0.05),COX-2、ICAM-1在肿瘤近端与肿瘤远端组织的表达差异无统计学意义。结论肿瘤组织与肿瘤近端、肿瘤远端之间密切相关,肿瘤组织即使被切除,但在肿瘤近端、远端组织中仍然存在促进肿瘤复发转移的物质基础,肿瘤术后防复发转移关键在于改善术后机体的微环境。

Objective After treatment of gastric cancer such as surgery,tumor is easy to recurrence or metastasis,detect tumor-associated factor expression in tumor tissue,proximal tissue away from tumors and distal tissue away from tumors to clear the importance of improving the residual stomach or body microenvironment of the tumor-prone.Methods After orthotopic,take MKN-45 gastric cancer tissues,proximal tissue away from tumors and distal tissue away from tumors.MaxVision two-step immunohistochemical detection VEGF,KDR,E-Cad,COX-2,telomerase,ICAM-1 gene protein expression.Results The tumor tissue and proximal tissue away from tumors and distal tissue away from tumors VEGF,KDR,E-Cad,COX-2,ICAM-1,telomerase gene protein expression differences were statistically significant (P<0.01),proximal tissue away from tumors and distal tissue away from tumors VEGF,KDR gene protein expression were significantly different (P<0.01),E-Cad,telomerase expression were significantly different(P<0.05),COX-2,ICAM-1 in distal tissue away from tumors were no statistically significant.Conclusions There was close relationship among the tumor tissue and proximal tissue away from tumors,distal tissue away from tumors.In the proximal and distal tissues away from tumors,there are still the material basis of promoting tumor recurrence even if the tumor was removed.The key to prevent tumor recurrence and metastasis is to improve the microenvironment of the body after surgery.