继发性输尿管梗阻对大鼠肾盂输尿管交界处ICC样细胞的影响

The effects of artificial ureteral obstruction on interstitial cell of Cajal-like cells in rats

目的探讨继发性输尿管梗阻后大鼠肾盂输尿管交界处ICC样细胞形态和密度的变化规律。方法清洁级SD大鼠35只,随机分为7组,每组5只。大鼠被分为三个大组,5只作为正常对照组(C),5只作为假手术组(S),其余作为实验组:梗阻3d组(S1)、梗阻1周组(S2)、梗阻2周组(S3)、梗阻4周组(S4)、梗阻8周组(S5)。实验组动物建立左侧输尿管完全梗阻模型。取肾盂输尿管交界处上下各0.2cm输尿管采用免疫组织化学显色,光镜下计数ICC样细胞密度并观察形态。结果所有实验组大鼠左肾盂及输尿管均见明显扩张积水,动物模型建立成功。各组ICC样间质细胞的密度分别为:C(7.20±2.29)个/视野,S(7.02±2.68)个/视野,S1(3.54±1.85)个/视野,S2(6.30±1.19)个/视野,S3(4.30±0.67)个/视野,S4(4.42±1.45)个/视野,S5(4.70±1.09)个/视野。除了S2组以外的各实验组ICC样间质细胞的密度较正常对照组(C)和假手术组(S)有所下降(P均<0.05),但呈现出先下降后上升再下降的趋势,这种差异在各个实验组之间无统计学意义。结论大鼠肾盂输尿管交界处ICC样间质细胞的密度变化与输尿管蠕动活性关系密切,为将来研究ICC样间质细胞在泌尿系统中的作用奠定了一定的基础。

Objective To determine the changes in density and morphology of interstitial cells of Cajal(ICC)-like cells(ICC-LC) at the ureteropelvic junction(UPJ) of rats after artificial distal ureteral obstruction.Materials and Methods Of a total of 35 rats,5 served as controls(C),5 underwent sham-operations(SH) and 25 were in the study(S) groups.The UPJs were extracted initially in the C and SH groups,and 3,7,14,28,56 days after ligation of the distal ureter in the study groups(S1,S2,S3,S4 and S5,respectively).The sections stained by c-kit anticore were studied under a light microscope.Results Ureterohydronephrosis was observed in all the rats after the obstruction was established.The mean number of ICC-LC was(7.20±2.29)/HP in C,(7.02±2.68)/HP in SH,(3.54±1.85)/HP in S1,(6.30±1.19)/HP in S2,(4.30±0.67)/HP in S3,(4.42±1.45)/HP in S4,and(4.70±1.09)/HP in S5.There was a statistically significant decrease in ICC-LC density in the study groups,except S2,compared to the C and SH groups.No significant difference was detected in Cajal cell morphology between groups.Conclusions Based on the changes in density of ICC-LC at the UPJ after obstruction of the distal ureter,we suggest that ICC-LC have a close relationship with motility of the ureter.