背根神经节脉冲射频对坐骨神经分支结扎切断模型大鼠脊髓甲硫氨酸脑啡肽和P物质的影响

The effect of pulsed radiofrequency on dorsal root ganglion on the met-enkephalin and substance P levels in the spinal cord in spinal nerve injury rats

目的对坐骨神经分支结扎切断模型(SNI)大鼠的背根神经节进行脉冲射频,通过放射免疫方法检测大鼠脊髓甲硫氨酸脑啡肽(M-ENK)、P物质水平;探讨背根神经节脉冲射频镇痛作用的可能机制,为临床应用提供理论基础。方法清洁级SD大鼠48只随机分为6组:正常动物组,SNI痛模型组,SNI+假手术后2h组,SNI+假手术后24h组,SNI+脉冲射频后2h组,SNI+脉冲射频后24h组。分别于脉冲射频后2h和24h测定大鼠脊髓M-ENK、P物质的含量。结果 (1)SNI+脉冲射频后2h大鼠脊髓M-ENK水平下降(P<0.01),24hM-ENK水平上升(P<0.01);(2)与正常动物组相比,SNI术后各组大鼠脊髓P物质的含量上升(P<0.01);与SNI模型组、SNI+假手术组相比,SNI+脉冲射频后2h及24h组大鼠脊髓P物质的含量上升(P<0.01)。结论 SNI模型后大鼠脊髓P物质的含量上升,脉冲射频可以增高大鼠脊髓M-ENK及P物质的表达水平。

Objective Applied pulsed radiofrequency on dorsal root ganglion of spared nerve injury(SNI) rats then examined the changes of met-enkephalin(M-ENK) and substance P(SP) levels in the spinal cord.The aim of this study is to find the possible mechanism of pulsed radiofrequency in the treatment of neuropathic pain and provide evidence for clinical applying.Methods Forty-eight male SD rats were randomly divided into six groups:the normal group(n=8),the SNI group(n=8),the 2 h after sham operation group(n=8),the 2 h after pulsed radiofrequency group(n=8),the 24 h after sham operation group(n=8),and the 24 h after pulsed radiofrequency group(n=8).The M-ENK and SP levels in spinal cord were examined by radioimmunoassay 2 h or 24 h after pulsed radiofrequency or sham operation.Results Two hours after the pulsed radiofrequency,the M-ENK levels decreased significantly.However,24 h after the pulsed radiofrequency,the M-ENK levels significantly increased.The SP level increased significantly after the SNI procedure,and were examined further increase at the 2 h and 24 h after pulsed radiofrequency.Conclusions This study demonstrated that applying pulsed radiofrequency can increase the endogenous M-ENK and SP levels in spinal cord in rat SNI model,this was involved in the effect of pulsed radiofrequency on neuropathic pain therapy.