应用RAPD技术探讨木兰科10属17种植物的遗传多样性

Genetic Diversity among 10 Genera 17 Species of Magnoliaceae by RAPD Marker

目的:采用RAPD技术对木兰科10属17种植物进行遗传多样性分析。方法:采用改良CTAB法提取基因组DNA,用筛选的9个引物的RAPD结果进行聚类分析,得出17种木兰科植物的聚合树状图。结果:9条RAPD引物共扩增出346条清晰稳定的条带,其中有295个多态性位点,多态百分比为85.3%,聚类分析表明,在遗传距离为0.728处17个供试品种分为3个类群。结论:中国木兰科植物品种有丰富的遗传多样性,RAPD技术可以用于木兰科植物的种间鉴别和遗传学研究。

Objective:The random amplied polymorphic DNA(RAPD) technique was applied to assess the genetic diversity among 10 genera 17 species of Magnoliaceae.Method:Total DNA was extracted by modified CTAB method.Based on the cluster analysis of their RAPD strips which were amplified with nine oligonucleotide(10 mer) primers which were previously selected,the molecular phylogenetic tree of 17 Magnoliaceae samples was described.Result:A total of 346 clear and reproducible bands were generated using nine oligonucleotide(10 mer) primers,and 295 polymorphic fragments(85.3%)were detected.Cluster analysis showed that,when the genetic distance was 0.728,the forms tested were divided into three groups.Conclusion:These indicated that there was abundant genetic diversity in species of Magnoliaceae of China.RAPD molecular marker may be of value for the authentication of different species and genetic diversity analysis of Magnoliaceae.