毒死蜱分子印迹聚合物的制备与性能研究

Research on the Preparation and Performance of Chlorpyrifos' Molecularly Imprinted Polymers

分子印迹技术是近些年发展起来的一种新技术,因其具有构效预定性、特异识别性和广泛实用性等特点而被广泛应用于环境、生物医药等领域,其在食品安全检测中也有重要的研究潜力。以毒死蜱为模板分子,采用原位逐步聚合法制备了具有良好识别性能的分子印迹聚合物(MIPs),考察了毒死蜱、敌敌畏及甲胺磷在毒死蜱分子聚合物上的选择性分离富集特性。用毒死蜱分子聚合物固相萃取了蜂蜜、蔬菜和天然水中的毒死蜱。结果表明,聚合物对模板分子产生了印迹效应,对毒死蜱有明显的选择性,流速为1.0 mL.min-1,进样量为20μL,加样回收率为96%～102%,RSD小于6.6%。该方法操作简单,针对性强,不需复杂的样品前处理,可用于食品中毒死蜱残留量的测定。

As a new technique of recent development,molecular imprinting technique(MIT) with predetermination,specific recognition and practicability is widely used in the domain of environment,biological medicines,and so on.It also has important research potential in food security test.In this paper,using chlorpyrifos as template,the molecular imprinted polymers was prepared by in situ step-addition polymerization.The characteristics of selective separation and pre-concentration for chlorpyrifos,dichlorovos and methamidophos were examined on the polymer.The chlorpyrifos from the honey,vegetables and natural water was extracted via using the polymer.The extraction conditions of MISPE column for chlorpyrifos were optimized.The results indicated that different kinds of recognition sites in the MIPs showed specific affinity toward chlorpyrifos.Velocity is 1.0 mL·min-1,the sampling volume of 20 μL.Average recoveries for chlorpyrifos in a blank sample spiked at 3 levels were between 96.0 %and 102%,with a RSD of less than 6.6%.This method is easily operated,highly selective and has no requirement of complicated sample pretreatment and is applicable for the determination of chlorpyrifos residue in foods.