连翘提取物LQ-4对SGC-7901胃癌细胞体外促凋亡作用研究

Research on forsythia anti-tumor component(LQ-4) effect on apoptosis of SGC-7901 cells in vitro

目的观察连翘提取物LQ-4对SGC-7901胃癌细胞体外促凋亡作用,为LQ-4在抗肿瘤机制研究及应用提供理论依据。方法应用MTT法测定LQ-4对SGC-7901胃癌细胞增殖的抑制作用;采用AO-EB染色、透射电镜、流式细胞仪检测其促凋亡作用。结果 LQ-4对SGC-7901胃癌细胞体外增殖抑制呈明显的剂量时间依赖性,LQ-4处理细胞6、12、24h后的半数抑制浓度(IC50)分别为(73.27±3.19)μg/ml、(44.63±2.06)μg/ml、(35.99±2.43)μg/ml,各组间差异有统计学意义,F=15.25(P<0.01);AO-EB染色表明药物组细胞出现明显凋亡,阴性对照组细胞形态完好;透射电镜表明,SGC-7901胃癌细胞在LQ-4处理后出现典型凋亡细胞形态改变;FCM分析结果表明,SGC-7901胃癌细胞在LQ-4处理前后细胞凋亡率发生改变,其中溶媒对照组为(0.73±0.49)%,25、50、100μg/mlLQ-4作用组分别为(8.04±0.68)%、(18.45±0.83)%、(52.37±0.74)%,各组间差异有统计学意义,F=13.17(P<0.05)。结论连翘提取物LQ-4体外能明显抑制SGC-7901胃癌细胞增殖并诱导其发生凋亡,具有明显的抗肿瘤作用。

Objective To investigate the apoptosis of SGC-7901 cells induced in vitro by LQ-4 which was extracted from forsythia,and investigate the relevant molecular mechanism.Methods SGC-7901 cells were treated with various concentrations of LQ-4 for 24 h,and cells were observed under inverted microscope.The inhibitory rate of cell proliferation was measured by MTT.The morphological changes effect of LQ-4-induced apoptosis was assessed by AO/EB fluorescent staining and transmission electron microscopy(TEM).The apoptosis rate was examined by flow cytometry(FCM).Results Under inverted microscope,the proliferation of cells decreased with the increasing concentration of the LQ-4.The same results were obtained by MTT,different concentrations of LQ-4(25-100 μg/ml) at different time intervals(6 h,12 h,24 h) had inhibitory effect on the proliferation of SGC-7901 cells in a time-dose-dependent manner.The result of AO/EB double staining showed significant apoptosis in exposed drug group but intact cell morphology in negative control group.TEM showed the typical morphological features of nuclear condensation and fragmentation in SGC-7901 cells after exposure to LQ-4.The scatter diagram of FCM showed that the cell viabilitu decreased with LQ-4 dose increasing,and the cell viability of the 100 μg/ml group was the lowest.There was significant difference of percentages of apoptotic cells among groups.Conclusions These results suggest that LQ-4 has the effects of inhabiting growth and inducing apoptosis in human stomach cancer cell line SGC-7901 in vitro.