姜黄素对β淀粉样蛋白诱导的老年性痴呆大鼠小胶质细胞活化的影响

Effects of curcumin on microglia activation in Alzheimer disease rat by injecting Aβ_(1-40)

目的探讨姜黄素对β淀粉样蛋白(Aβ)诱导的老年性痴呆(AD)大鼠学习记忆障碍及小胶质细胞活化的影响。方法将Aβ1-40微量注射至大鼠右侧海马,制作AD大鼠模型,干预组给予腹腔注射姜黄素连续7d,造模1个月后进行Morris水迷宫试验测定大鼠学习记忆能力,分为空白对照组、对照组和姜黄素给药组。免疫组织化学方法检测小胶质细胞。结果 AD对照组大鼠较空白对照组逃避潜伏期延长,而跨越原平台位置次数明显减少;姜黄素给药组大鼠较AD对照组第2～5天平均逃避潜伏期明显缩短,与空白对照组比较无明显差异(P>0.05),跨越原平台位置次数明显增多,与空白对照组无明显差异(P>0.05),表明姜黄素干预后AD大鼠空间学习、记忆能力明显改善。与空白对照组相比,AD对照组大鼠脑组织海马区可见小胶质细胞表达明显增多(P<0.05);姜黄素组大鼠脑组织海马区小胶质细胞较AD对照组明显减少(P<0.05)。结论姜黄素能够改善Aβ1-40诱导的AD模型大鼠空间学习记忆障碍,其机制可能与抑制Aβ所致小胶质细胞活化有关。

Objective To explore effects of curcumin on learning and memory impairment and microglia activation in Alzheimer disease rat. Methods Twenty-four rats were randomly divided into sham-operation group (SC),AD model group (AD) and curcumin treatment group (C).Aβ1-40 was microinjected into the right hippocampus of rats.The treatment group received curcumin intraperitoneal injection lasted for 30 days.Morris water maze was used to study the learning and memory ability of action,and immunohistochemistry was adopted to assess microglia in the hippocampus.Results The mean escape latency in AD group was obviously increased and the frequency of passing through the platform was visibly decreased compared with the sham operated group;the mean escape latency of curcumin group was obviously decreased on day 2-5 of place navigation test and the frequency of passing through the platform was visibly increased compared with the AD group.The number of microglia was increased significantly in the hippocampus of the AD group compared with the sham operated group;the number of microglia was decreased significantly in the hippocampus of the curcumin group compared with that in the AD group.Conclusions Curcumin could inhibit microglia activation induced by Aβ1-40 and improve learning and memory ability in AD rat.