神经导管结合神经片段修复外周神经损伤

Study of using nerve conduits containing with nerve fragments to repair rat's peripheral nerve defect

目的研究神经片段结合神经导管治疗外周神经缺损,为周围神经损伤修复与重建的解决途径提供实验依据。方法 24只成年Wistar大鼠随机分为A、B、C组,分别为神经自体移植、与含有神经片段的神经导管和单纯导管移植桥接大鼠10mm神经缺损,各组间在形态学、电生理、组织学进行比较。结果 B组大鼠移植侧肢体足迹实验的坐骨神经功能指数(SFI)为-81.20±2.81,神经干传导速度为(28.93±5.07)m/s、动作电位的振幅为(5.46±2.76)mV,再生神经中段检查髓鞘化轴突密度为(3953±468)/视野,髓鞘面积为(2.77±0.83)μm,与C组比较差异有统计学意义(P<0.05)。结论含有神经片段的神经导管组织工程移植体桥接神经缺损有促进神经轴突再生与神经功能恢复的良好作用,效果优于单纯神经导管移植。

Objective To study the effect of using nerve conduits containing with nerve fragments to repair rat's peripheral nerve defect,and to provide theory foundation and experiment experience for further study on repair and regeneration of peripheral nerve defect. Methods 24 Wistar rats with 10 mm sciatic nerve defect of the right lower extremity were divided into three groups: the nerve defects of group A were repaired with autochthonous graft, group B with nerve conduits containing of nerve fragments and group C with nerve conduits only. Morphology and functional rehabilitation were observed and electrophysiology tests of rats were performed. Results The results of walking track analysis:SFI:-81.20±2.81, electrophysiology tests [NCV:(28.93±5.07) m/s,AMP:(5.46±2.76)mv] and histology test of regeneration nerves [myelinization density:3953±468/HP,myelin sheath area:(2.77±0.83)μm] on rats in group B were significantly better than those in group C (P< 0.05), and similar with those in group A (P>0.05). Conclusions The effect of using nerve conduits containing nerve fragments is better than using nerve conduits only, especially for neurite regeneration and functional rehabilitation, although the effect may not be as well as autochthonous graft.