异甘草酸镁联合脾切除对大鼠肝切除后早期肝衰竭的治疗作用

Effect of administration of magnesium isoglycyrrhizinate combined with splenectomy on a rat model of acute hepatic failure induced by major liver resection

目的探讨异甘草酸镁联合脾切除在大鼠肝切除术后早期肝衰竭中的作用。方法将50只SD大鼠随机分为85%肝切除对照组、脾切除处理组、异甘草酸镁处理组、脾切除加异甘草酸镁处理组和假手术对照组。异甘草酸镁处理组和脾切除加异甘草酸镁处理组腹腔内注射异甘草酸镁(100μg/ml)1ml/100g,其余各组腹腔内注射等量生理盐水。术后观察各组大鼠血清ALT、AST变化、门静脉压力变化及肝组织的病理学改变。结果各处理组的ALT、AST、门静脉压力均较肝脏手术切除对照组明显降低(P<0.05);但异甘草酸镁处理组较肝切除对照组门静脉压力下降无明显统计学意义(P>0.05)。结论异甘草酸镁联合脾切除可能通过降低门静脉压力,减轻残肝细胞损害对大鼠85%肝切除术后早期肝衰竭产生治疗作用。

Objective To investigate the effect of administration of magnesium isoglycyrrhizinate combined with splenectomy on rats with subtotal hepatectomy. Methods 50 SD rats were randomly divided into five groups:85% hepatectomy control group (group A), 85% hepatectomy with splenectomy group (group B),treated with magnesium isoglycyrrhizinate group (group C), treated with magnesium isoglycyrrhizinate combined with splenectomy group (group D) and the sham operation group(group E). The rats in group C and D were intraperitoneally injected with magnesium isoglycyrrhizinate (100 μg/ml)1 ml/100 g,while the other groups injected with normal saline. The changes of plasma ALT and AST, portal venous pressure were recorded and the liver pathological examination were screened post-operation. Results The ALT and AST and portal venous pressure in treatment groups were significantly lower than those in 85% hepatectomy group at 24 h and 48 h post-operation(P<0.05). But there were no such differences between group A and group C(P>0.05). Conclusions Administration of magnesium isoglycyrrhizinate combined with splenectomy might prevent the acute hepatic failure induced by 85% hepatectomy on rat,and its possible mechanism might relate to the decreasing portal vein pressure and alleviating the residual hepatic cell injury.