miR-345在胃癌中的表达特点及其临床意义

Expression and its clinical significance of miR-345 in human gastric cancer

目的探讨miR-345在胃癌中的表达特点及其临床意义。方法采用实时荧光定量逆转录聚合酶链式反应检测人胃黏膜上皮细胞GES-1、3株胃癌细胞系(SGC7901、MGC803、MKN45)及48例配对的胃癌与癌旁组织中miR-345的表达水平,并对miR-345表达水平与患者临床病理特征、生存预后的关系进行相关性分析,最后通过PicTar和TargetScan软件预测miR-345潜在的重要靶基因。结果与正常组织比较,胃癌细胞系及胃癌组织中miR-345表达明显升高(P<0.05);miR-345的表达与胃癌的分化程度(P=0.005),TNM分期(P=0.003),原发肿瘤的浸润深度(P<0.001)和淋巴结转移(P<0.001)密切相关;过度表达的miR-345预示胃癌患者的术后生存期更短;miR-345潜在的重要靶基因为CDKN1A、BBC3和RUNX3。结论 miR-345与胃癌的发生、发展密切相关,可能成为胃癌诊治和评估预后的潜在生物学指标。

Objective To investigate the expression of miR-345 in human gastric cancer and its clinical significance.Methods By quantitative real time reverse transcription-PCR,expression of miR-345 in normal gastric mucosa epithelial cells and gastric cancer cell lines,paired gastric cancer tissues and adjacent tissues were detected.Correlation analysis was applied to illustrate the relationship between the level of miR-345 and the clinical-pathological features or survival prognosis in 48 patients.Potential targets of miR-345 were searched by the Pictar and Targetscan prediction program.Results Real time PCR analyses revealed that miR-345 expressions were markedly upregulated in gastric cancer cell lines and tissues.Further statistical analyses showed that miR-345 level was strongly associated with differentiation (P=0.005),TNM stage(P=0.003),depth of invasion (P<0.001) and lymph node status(P<0.001);the survival time of patients with high miR-345 expression being poorer;CDKN1A,BBC3 and RUNX3 may become potential target genes of miR-345.Conclusions The level of miR-345 might play an important role in human gastric cancer,and thereby might be a novel and useful prognostic marker and a potential target for human gastric cancer treatment.