摘要
目的探讨大鼠分泌性中耳炎中耳微环境中的Th2/Th1细胞失衡模式。方法选用SD大鼠20只,随机分为实验组和对照组,每组各10只(20耳)。实验组以卵清蛋白腹腔致敏后耳内激发制成分泌性中耳炎(OME)模型,对照组以PBS替代卵清蛋白。耳内激发后2d处死动物,采用HE染色切片观察各组中耳黏膜病理变化及炎症细胞的改变,采用ELISA法测定各组大鼠中耳腔灌洗液中Th2型细胞因子白介素4(IL-4)和Th1型细胞因子干扰素γ(IFN-γ)的含量,免疫组化染色检测中耳黏膜和骨髓腔中IL-4、IFN-γ的表达。结果实验组与对照组比较中耳灌洗液中IL-4含量、Th2/Th1(IL-4/IFN-γ)比值以及中耳黏膜和骨髓腔中IL-4表达、Th2/Th1(IL-4/IFN-γ)比值均出现一致性增高,差异均有统计学意义(P<0.05);而中耳微环境中IFN-γ含量组间差异无统计学意义(P>0.05)。结论在变应原刺激下OME大鼠中耳微环境中IL-4合成显著增高,而IFN-γ相对减少,Th2/Th1比值增高,存在以Th2亢进为特征的Th2/Th1细胞失衡反应,中耳微环境的Th2/Th1细胞失衡模式是分泌性中耳炎重要的免疫学发病机制之一。
Objective To investigated the shift of Th2/Th1 type cytokine profile in middle ear of rat model of otitis media with effusion (OME).Methods Twenty SD rats were randomly divided into OME (Exp group) and control group (Con group),OME was induced by intraperitoneal injection of OVA and later challenging in tympanic bullae.The expression of IFN-γ and IL-4 were observed by immunohistochemistry in temporal bone marrow cavity and middle ear mucosa.The level of IFN-γ and IL-4 in tympanic lavage fluid (TLF) were determined by ELISA.Results Pathological examination showed that middle ear mucosa inflammation and eosinophil infiltration in Exp group were obviously severe than Con group.As compared to the Con group,the level of IL-4 and the ratio of Th2/Th1(IL-4/IFN-γ)significantly increased(P<0.05) in TLF,temporal bone marrow cavity and middle ear mucosa of Exp group,however,no significant difference in IFN-γ levels was found(P>0.05) between Exp and Con group.Conclusions Th2-cytokine profile predominates in the middle ear microenvironment of allergic OME rat model.Th1/ Th2 imbalance is involved in the pathogenesis of OME.
出处
《中华临床医师杂志（电子版）》
CAS
2011年第24期7282-7286,共5页
Chinese Journal of Clinicians(Electronic Edition)
基金
国家自然科学基金(81070777)
