细胞骨架及运动基因对瘢痕挛缩作用的实验研究

Effect of fibroblast cytoskeletal gene expression on the contraction of hypertrophic scar

目的:探讨细胞骨架和运动相关基因与瘢痕挛缩的关系以及这些基因间的相互作用。方法:收集病人不同时期增生与扁平瘢痕作为研究标本,利用基因芯片筛选结果选择表达最强、涉及细胞结构主要方面的5个基因,制成寡核苷酸探针与瘢痕组织切片原位杂交。同时,将各标本进行组织块培养、成纤维细胞爬片、原位杂交。结果:各种基因在早期增生性瘢痕中表达均明显强于晚期增生及扁平瘢痕;其中,α-平滑肌肌动蛋白出现早、持续时间长、表达明显强于其他基因;更有意义的是,它在增生性瘢痕与扁平瘢痕之间表达差异显著大于其他。结论:瘢痕增生挛缩与细胞骨架运动的各方面相关基因存在密切关系,而平滑肌肌动蛋白起到关键与首要的作用。同时,各种基因参与其启动与形成,抑制该基因的表达,可能实现减轻瘢痕挛缩。

Objective:To investigate the effect of fihroblast cytoskeletal gene expression on the formation and contraction of hypertrophic scar and relationship among these genes.Method:According to the results of different- ly expressed genes in hypertrophic sear by microarray,five of the most important genes were selected and made in- to oligonucleotide probes.24 cases of hypertrophic scar,8 non-hypertrophic scars and 4 normal skins were used, and these scars were harvested 3,6,9,or 12 months after burns.Frozen sections and cultured fibroblasts were made to detect the expression of these genes by in situ hybridization.Results;The expression of five genes were found in sear tissue,but that in hypertrophic scars was significantly stronger than non-hypertrophic scars.Alpha- smooth muscle actin showed the strongest expression among five genes.Conclusions:Over-expression of cytoskele- tel relative genes might cause the contraction of scars,and alpha-sma seem to play a leading and key role in hyper- trophic scar formation.