增生性瘢痕组织角质形成细胞中神经丝蛋白表达的特征及生物学意义

The biologic significance of NFP expression in epithelia basal keratinocyte of hyperplastic scars

目的:观察瘢痕组织与成人正常皮肤上皮细胞中神经丝蛋白(NFP)及其表皮细胞生长因子受体(EGFR)表达变化,阐明神经再支配对增生性瘢痕形成的影响。方法:标本取自烧伤瘢痕愈合后11～26月来我院进行修复手术的病人,正常对照选自同一病人的供皮区。利用单克隆抗体免疫组织化学染色技术,光镜下观察瘢痕组织及正常皮肤上皮细胞中NFP和EGFR阳性细胞表达情况。结果:瘢痕组织与正常皮肤上皮中神经丝蛋白与表皮细胞生长因子受体的表达存在差异,随着瘢痕的成熟,神经丝抗体的表达逐渐增强,而瘢痕早期表皮细胞生长因子受体的表达较强,瘢痕后期的表达减弱。结论:增生性瘢痕与正常的皮肤中NFP的变化与EGFR活性与创面愈合的结局密切相关。神经调节在瘢痕形成的过程中可能充当了重要的角色。

Objective:To investigate the implication of neurofilament proteins (NFP) and epidermal growth factor receptor (EGFR) expression in epithelial cells of hyperplastic scars and healthy skins, in an attempt to explain the pathogenesis of scar formation. Methods:The samples of scar were obtained from post-burn (11-26 months) patients undergoing plastic operation in our burn unit recently, and the samples of control came from skin donor site of the same patient respectively. Immunohistochemistry staining technique with monoclonal antibody was employed and the expression of both neurofilament proteins (NF-200) and epidermal growth factor receptor (EGFR) in keratinocytes was examined under light microscope. Altogether 6 hyperplastic scars and 6 normal skin specimen were studied. Results: Evident difference in the NFP and EGFR positive keratinocytes of skin was found between hyperplastic scars and normal skin. The NF-200 and EGFR expressions were stronger in hyperplastic scars than that in normal skin, and NF-200 immuno-reaction positive cells were progressively increased with scar maturation, and in contrast, the expression of EGFR became progressively weaker. Conclusion:It is our preliminary assertion that the activity of epidermal growth factor receptor (EGFR) might bear a relationship with reinnervation, and the expression of EGFR might influence wound healing. Therefore, neuroregulation might play a role in the pathogenesis of hyperplastic scars.