肠缺血再灌流时肠内给予葡萄糖能增加肠粘膜血流量

Enteral glucose can augment mucosal blood flow during gut ischemia/reperfusion

目的:探讨肠缺血再灌流损伤时肠内营养与肠粘膜血流改变的关系。方法:SD大鼠分为三组,先制作空肠袋,然后将激光多谱勒探头和肠粘膜张力计放置在空肠袋两端,分别向袋内注射丙氨酸、葡萄糖及甘露醇。用动脉夹阻断肠系膜上动脉血流60分后,再恢复灌流60分。分别测定肠粘膜血流量和局部PCO_2张力(PrCO_2)。结果:缺血再灌流过程中,与甘露醇组比较,葡萄糖组粘膜血流量显著增加,PrCO_2降低。结论:缺血再灌流过程中,肠内给予葡萄糖能增加肠粘膜血流量,对缺血再灌流损伤的肠道提供保护作用。

Objective:To determine if the specific nutrients are associated with alterations in mucosal perfusion during gut ischemia/reperfusion. Methods:Sprague Dawley rats had either a laser doppler probe or a tonometer inserted into a jejunal sac filled with either 10 mM alanine, glucose or mannitol (osmotic control) followed by 60 minutes of superior mesenteric artery occlusion and 60 minutes of reperfusion. Laser doppler mucosal blood flow and regional PCO_2 (PrCO_2) measurements were obtained. Results: Mucosal blood flow was significantly increased during both ischemia and reperfusion when intraluminal glucose was present as compared to intraluminal alanine. Blood flow changes were reflected by lower jejunal PrCO_2 measurements with intraluminal glucose. Conclusions: Intraluminal glucose can augment mucosal blood flow during gut ischemia/reperfusion I/R and may provide the protective effect on gut from ischemia and reperfusion injury.