hiv/aids合并早期隐性梅毒患者T淋巴细胞及激活亚群表达

expression of t lymphocytes and activated subsets in patients with hiv/aids and early latent syphilis

目的 研究hiv/aids合并早期隐性梅毒患者外周血t淋巴细胞及其激活亚群的变化,探讨其临床意义.方法 用流式细胞仪检测78例hiv/aids患者、66例hiwaids合并早期隐性梅毒患者和30名健康体检者的t淋巴细胞及其激活亚群( hla-dr+ cd3 +/cd3+、hla-dr+cd4 +/cd4+和hla-dr+ cd8 +/cd8+),同时行梅毒快速血浆反应素试验(rpr)和梅毒螺旋体明胶凝集试验(tppa)检测.采用spss 13.0软件进行统计分析,两样本均数的比较采用t检验(正态分布)或mann-whitney u检验(偏态分布).结果 hiv/aids合并梅毒患者cd4+t淋巴细胞计数显著高于hiv/aids组(t=2.041和2.223,p值均＜0.05),但在cd3+t和cd8 +t淋巴细胞计数方面差异无统计学意义(tcd3 =0.362和0.692,tcd8=0.043和0.617,p值均＞0.05).aids合并梅毒组hla-dr+cd4+/cd4+显著高于hiv合并梅毒组(t=2.647,p＜0.05),但在hla-dr+ cd3 +/cd3+和hla-dr+cd8 +/cd8+方面两组比较差异无统计学意义(t=1.112和0.093,p值均＞0.05).结论 hiv/aids患者感染隐性梅毒早期细胞免疫功能存在一过性增强的可能. abstract： objective to investigate the expression of t lymphocytes and activated subsets in patients with hiv/aids and early latent syphilis. methods t lymphocytes and activated subsets ( hladr+ cd3 +/cd3 + , hla-dr+ cd4 +/cd4 + and hla-dr+ cd8 +/cd8 + ) as well as rapid plasma reagin (rpr) and treponema pallidum particle agglutination (tppa) test were detected by flow cytometry in 78 patients with hiv/aids, 66 patients with hiv/aids and early latent syphilis, and 30 healthy subjects. spss 13.0 was used for statistical analysis, and t (for normal distribution) or mann-whitney u (for skew distribution) tests were performed to compare between the groups. results the absolute counts of cd4+ t cells in patients with hiv/aids and early latent syphilis were significantly higher than those in hiv/aids patients ( t = 2. 041 and 2. 223, p ＜ 0.05 ), but no difference in the counts of cd3 + t cells and cd8 + t cells was observed (tcd3 =0. 362 and 0.692, tcd8 =0.043 and 0.617, p＞0.05). hla-dr+ cd4 +/cd4 +level in aids plus syphilis group was much higher than that in hiv plus syphilis group ( t = 2. 647, p ＜ 0. 05 ), but no difference was observed in hla-dr+ cd3 +/cd3 + and hla-dr+ cds +/cds + ( t = 1. 112 and 0. 093, p ＞ 0.05). conclusions immune function in patients with hiv/aids and early latent syphilis may be enhanced temporarily.

Objective To investigate the expression of T lymphocytes and activated subsets in patients with HIV/AIDS and early latent syphilis. Methods T lymphocytes and activated subsets ( HLADR+ CD3 +/CD3 + , HLA-DR+ CD4 +/CD4 + and HLA-DR+ CD8 +/CD8 + ) as well as rapid plasma reagin (RPR) and treponema pallidum particle agglutination (TPPA) test were detected by flow cytometry in 78 patients with HIV/AIDS, 66 patients with HIV/AIDS and early latent syphilis, and 30 healthy subjects. SPSS 13.0 was used for statistical analysis, and t (for normal distribution) or Mann-Whitney U (for skew distribution) tests were performed to compare between the groups. Results The absolute counts of CD4+ T cells in patients with HIV/AIDS and early latent syphilis were significantly higher than those in HIV/AIDS patients ( t = 2. 041 and 2. 223, P ＜ 0.05 ), but no difference in the counts of CD3 + T cells and CD8 + T cells was observed (tcD3 =0. 362 and 0.692, tcD8 =0.043 and 0.617, P＞0.05). HLA-DR+ CD4 +/CD4 +level in AIDS plus syphilis group was much higher than that in HIV plus syphilis group ( t = 2. 647, P ＜ 0. 05 ), but no difference was observed in HLA-DR+ CD3 +/CD3 + and HLA-DR+ CDs +/CDs + ( t = 1. 112 and 0. 093, P ＞ 0.05). Conclusions Immune function in patients with HIV/AIDS and early latent syphilis may be enhanced temporarily.