广州地区儿童偏肺病毒感染的分子流行病学研究

molecular epidemiological study on human metapneumovirus infection among children in guangzhou

目的 了解广州地区婴幼儿患者呼吸道中人偏肺病毒(hmpv)感染的分子流行病学特点.方法 采集2010年1-12月到广州市妇女儿童医疗中心就诊的呼吸道感染患儿的咽拭子等样本1840份,对临床样本进行hmpv检测,对f基因进行扩增,并对pcr产物进行测序.结果 1 840份临床样本中66份hmpv检测阳性,阳性率为3.59％.全年除9、10月份外每月均有hmpv检出,其中4月份检出阳性率最高,为6.09％.随机选取3株hmpv,将其f基因核苷酸序列与a1、a2、b1、b2、c型和2008年分离的hmpvgz01株的f基因序列进行clustal w比较,发现与a2b组的bj1887同源性最高,为97％,4株广州分离的病毒株之间f基因的同源性为99％.序列与进化树分析表明广州地区流行的是a2b型.结论 2010年广州地区hmpv流行季节是春夏季,广州地区流行株的型别为a2b亚型. abstract： objective to conduct a molecular epidemiological study on human metapneumovirus (hmpv) among pediatric patients in guangzhou. methods a total of 1 840 clinical specimens were obtained from pediatric patients with respiratory infections in guangzhou women and children' s medical center in 2010. hmpv was detected by real-time taqman rt-pcr in clinical specimens. f gene was amplified and the pcr-products were directly sequenced. results in 1 840 clinical specimens, 66 werehmpv-positive with a positive rate of 3.59％. hmpv was detected in all specimens except those collected in september and october, and the highest positive hmpv rate occurred in april (6.09％). the f genes of 3 randomly selected strains and hmpvgz01 ( isolated in 2008) were compared with subgroups a1, a2, b1,b2 and c, and the highest homology was with bj1887 strain of genotype a2b (97％). the f genes of the randomly selected strains and hmpvgz01 were 99％ identical to each other. sequences and phylogenetics analysis revealed that the epidemical strain in guangzhou belonged to genotype a2b. conclusion hmpv is prevalent in spring and summer among children in guangzhou, and a2b is the predominant genotype.

Objective To conduct a molecular epidemiological study on human metapneumovirus (hMPV) among pediatric patients in Guangzhou. Methods A total of 1 840 clinical specimens were obtained from pediatric patients with respiratory infections in Guangzhou Women and Children' s Medical Center in 2010. hMPV was detected by real-time TaqMan RT-PCR in clinical specimens. F gene was amplified and the PCR-products were directly sequenced. Results In 1 840 clinical specimens, 66 werehMPV-positive with a positive rate of 3.59％. hMPV was detected in all specimens except those collected in September and October, and the highest positive hMPV rate occurred in April (6.09％). The F genes of 3 randomly selected strains and hMPVgz01 ( isolated in 2008) were compared with subgroups A1, A2, B1,B2 and C, and the highest homology was with BJ1887 strain of genotype A2b (97％). The F genes of the randomly selected strains and hMPVgz01 were 99％ identical to each other. Sequences and phylogenetics analysis revealed that the epidemical strain in Guangzhou belonged to genotype A2b. Conclusion hMPV is prevalent in spring and summer among children in Guangzhou, and A2b is the predominant genotype.