多药耐药鲍曼不动杆菌adc型ampc酶基因研究

adc type cephalosporinase gene in multidrug-resistant strains of acinetobater baumannii

目的 了解多药耐药鲍曼不动杆菌(mdr-aba)中adc型ampc酶基因的存在状况.方法 收集2007年11月-2008年2月浙江大学医学院附属第一医院住院患者的临床样本中分离到的鲍曼不动杆菌62株.应用k-b纸片扩散法检测细菌对抗菌药物的敏感性.应用pcr及序列分析法分析adc型ampc酶基因.结果 62株mdr-aba中除对头孢哌酮/舒巴坦有较高敏感率(69.4%)以外,对其他抗菌药物的敏感率均较低.62株mdr-aba中检测到adc阳性36株(58.1%).结论 本组mdr-aba多药耐药情况十分严重,mdr-aba对β-内酰胺类抗菌药物耐药与其产adc型ampc酶密切相关. abstract： objective to investigate adc type cephalosporinase genes in muhidrug-resistant strains of acinetobater baumannii (mdr-aba). methods sixty-two aba strains were collected during november 2007 to february 2008 from the first affiliated hospital, college of medicine, zhejiang university. kirby bauer disk diffusion method was used to detect the susceptibilities of the strains to antimicrebial agents. pcr and dna sequencing was performed to analyze the adc type cephalosporinase gene. results sixty-two mdr-aba strains showed high drag-resistance rate to most antimicrobial agents expect cefoperazone/sulbactam (69.4%), and 36 strains were acd positive (58.1%). conclusion mdr-aba strains in this study are of high drug resistance, which is closely related to adc type cephalosporinase.