泛耐药鲍曼不动杆菌获得性耐药基因和可移动遗传元件检测与指标聚类分析

correlation between acquired drug resistance-related genes and mobile genetic elements in pandrugresistant acinetobacter baumannii

目的 调查泛耐药鲍曼不动杆菌获得性耐药基因和可移动遗传元件携带情况,并探讨二者之间的相关性.方法 收集临床样本中分离的20株泛耐药鲍曼不动杆菌,采用pcr法检测53种水平转移获得性耐药基因(与β-内酰胺类、氨基糖苷类、喹诺酮类耐药相关)以及12种可移动遗传元件(接合性质粒、转座子、插入序列、整合子等),并对检测结果进行指标聚类分析.结果 本组菌株检出3种β-内酰胺酶类药物耐药基因tem-1、adc-30和oxa-23,4种氨基糖苷类修饰酶基因aac(3)-Ⅰ、aac(6′)-Ⅰb、ant(3″)-Ⅰ和aph(3′)-Ⅰ,以及5种可移动遗传元件intⅠ1、tnpu、tnp513、is26和isaba1.指标聚类分析显示、tem-1、adc-30等2种β-内酰胺酶基因,aac (6′)-Ⅰb、ant(3″)-Ⅰ等2种氨基糖苷类修饰酶基因,abeb和qace Δ1外排泵基因与intⅠ1、tnpu、tnp513、is26、isaba1等可移动遗传元件高度相关.结论 临床分离的pdr-aba可携带多种获得性耐药基因和可移动遗传元件,且二者之间高度相关.

objective to investigate the correlation between acquired drug resistance-related genes and mobile genetic elements from pandrug-resistant acinetobacter baumannii. methods fifty-three horizontal transfer drug resistance-related genes ( β-lactamases,aminoglycoside and quinolones resistance related) and 12 mobile genetic elements (including zygosity plasmid,transposon,insertion sequence and integron) were detected by polymerase chain reaction (pcr) in 20 clinical isolates of pandrug-resistant acinetobacter baumannii.index cluster analysis was performed to explore the correlation.results in 20 strains of pandrug-resistant acinetobacter baumannii,there were 3 types of β-lactamases related genes (tem-1,adc-30,oxa-23 ),4 types of aminoglycoside modifying enzyme genes [ aac (3)-Ⅰ,aac(6′)-Ⅰ b,ant( 3″)-Ⅰ and aph( 3′)-Ⅰ ],and 5 kinds of mobile genetic elements ( int Ⅰ 1,tnpu,tnp513,is26 and isaba1 ). index cluster analysis showed high correlations between resistance genes [tem-1,adc-30,aac( 6′)-Ⅰ b,ant( 3″)-Ⅰ,abeb,qace Δ1] and mobile genetic elements ( int Ⅰ 1,tnpu,tnp513,is26,isaba1 ).conclusion clinical isolated pandrug-resistant acinetobacter baumannii carries several acquired drug resistance-related genes and mobile genetic elements,and there may be a close association between them.