反硝化菌株GW1的筛选及特性研究

Isolation and characteristics of denitrifying bacterium strain GW1

研究利用反硝化培养基,从实验室厌氧反硝化颗粒污泥中分离、筛选出1株反硝化优势菌株GW1,通过16SrDNA序列分析对其初步鉴定,并研究了温度、pH值、碳源、碳氮比和硝酸盐氮质量浓度对菌株GW1反硝化特性的影响。研究结果表明,菌株GW1的16SrDNA基因序列与Paracoccus versutus有最大相似性,达到99.9%,Genebank登录序列号为GU111570;分离菌株呈革兰氏阳性;最佳反硝化条件:丁二酸钠为碳源,温度为35～40℃,pH值为7.0～8.0,建议工程应用碳氮比为3∶1(质量比)。该菌株特性的研究为解决反硝化速率过慢问题提供了技术支持。

A highly active denitrifier named GW1 was isolated from the denitrification granule sludge,and 16S rDNA identification of the strain was performed.Influencing factors such as temperature,pH,carbon source and C/N ratio were optimized through batch experiments,and the effect of nitrate concentration on the denitrification was also studied.The phylogenetic analysis based on 16S rDNA suggests that strain GW1 is the closest relative of Paracoccus versutus with 99.9 % sequence similarity and high homology.Genbank sequence number is GU111570.Strain GW1 is Gram-positive.The optimal conditions for the denitrification by strain GW1 are sodium succinate as carbon source,temperature 35~40 ℃,pH(7.0~8.0)and proposed project use of C/N ratio 3,respectively.Strain GW1 can remove nitrate effectively.The study may help solve the problem of slow denitrifying rate.