苯溴马隆-Cu(Ⅱ)-牛血清白蛋白相互作用的荧光光谱法研究

Interaction of Benzbromarone with Bovine Serum Albumin:Investigation by Fuorescence Spectroscopy

应用荧光光谱法研究了生理条件下苯溴马隆对和Cu(Ⅱ)单独存在和同时存在对牛血清白蛋白荧光光谱特性的影响.结果表明:Cu(Ⅱ)和苯溴马隆均可使牛血清白蛋白的荧光强度发生静态猝灭,并且在Cu(Ⅱ)存在下,苯溴马隆对牛血清白蛋白的荧光猝灭作用显著增强.根据荧光猝灭双倒数图计算苯溴马隆和牛血清白蛋白的结合常数为9.54×105 L/mol,结合位点数为1.03;Cu(Ⅱ)与牛血清白蛋白之间的结合常数为1.13×103 L/mol,结合位点数为0.74.

Effects of benzbromarone(BXML) and Cu(Ⅱ) exist respectively or simultaneously on the fluorescence spectrum characteristics of bovine serum albumin(BSA)were studied.The results showed that both Cu(Ⅱ) and BXML could quench the fluorescence intensity of BSA via a nonradiative energy transfer mechanism.When Cu(Ⅱ) was present,BXML could quench the fluorescence of BSA more significantly.The binding constants(K) and the binding sites(n) were calculated by analyzing fluorescence quenching data with double-reciprocal equation.The K and n between BXML and BSA were 9.54×105L/mol and 1.03,those between Cu(Ⅱ) and BSA were 1.13×103 L/mol and 0.74 respectively.