人宫颈癌移植瘤乏氧动物模型的建立及鉴定

The Building and Evaluation of a Hypoxic -xenograft Animal Model of Human Cervical Cancer

目的 建立人宫颈癌移植瘤乏氧动物模型并进行鉴定。方法 人宫颈癌Hela细胞接种于裸小鼠右后大腿外侧皮下 ,每鼠 0 .2ml。待肿瘤长至瘤体溃烂前 (1.7cm左右 ) ,用双通道组织氧分压传感针测定肿瘤及正常组织的氧分压 ,取肿瘤组织作常规病理检查 ,并用免疫组化方法检测肿瘤组织乏氧诱导因子 1α表达。结果 (1) 8只裸鼠 ,7只成活 ,其中 7只成瘤 ,成瘤率 87.5 % (7/ 8)。瘤体平均大小1.75 85± 0 .2 0 2 0cm。 (2 )移植瘤乏氧状况测定 ,瘤体平均氧分压为 3.785 7± 0 .6 6 94kPa,明显处于乏氧状况 ,而作为对照的正常后腿氧分压为 11.7714± 2 .2 787kPa (t=7.6 78,P <0 .0 1)。 (3) 7只小鼠移植瘤的病理检查均为上皮性癌 ,HIF 1α表达均为强阳性 (+++)。结论 人宫颈癌移植瘤瘤体大小生长至瘤体溃烂前 (1.7cm左右 ) ,是一种较好与临床实际情况相符 ,适合研究需要 。

Objective To bulid a hypoxic animal model of the xenograft of human cervical carcinoma, and to evaluate this model.Methods We injected 0.2 ml solution of Hela cells into the lateral skin of right hindlimb of each nude mouse. When the xenograft is large enough (about 1.7 cm) to have the trend of ulcer, the partial tension of oxygen in the tumor and normal tissue was measured with bi passage transductive needle of tissue PO 2. The routine pathological examination of the xenograft was done, and the HIF 1α was detected with the methods of histoimmuniology.Results (1) 8 nude mice were used in this experiment, and 7 survived. 7 xenografts were in line with our demand, so the rate of successful neoplasm's growth was 87.5% (7/8). The mean size of xenografts was 1.7585 ± 0.2020 cm. (2) The hypoxic status of graft neoplasm. The mean PO 2 in xenograft was 3.3857 ± 0.6694 kPa, which proved an obvious hypoxia condition. The PO 2 of control group in the normal hindlimb was 11.7714 ± 2.2787 kPa. And t test showed that ( t = 7.678 , P < 0.01 ). (3) The pathological examination proved xenografts of 7 survived mice to be carcinoma and to have strong expression (+++) of HIF 1α in the xenografts.Conclusion When the xenograft is large enough (about 1.7 cm) to have the trend of ulcer, it can be used as a hypoxic animal model of xenograft, which is closed to the clinical status, available to the research and has high possibility of success.