人肾癌鸡胚模型的建立及其肿瘤生物学特性的研究

Establishment and biological characteristics of a human renal cell carcinoma model on chick embryo chorioallantoic membrane

目的建立人肾癌鸡胚模型,探讨其形态及生物学特性。方法将肾癌细胞系RLC-310接种于鸡胚绒毛尿囊膜,观察影响肾癌鸡胚移植瘤成活的因素、移植瘤生长特性、形态学特征和生物学性状。结果成功建立了人肾癌鸡胚移植瘤模型;移植瘤易于生长并具有较强的血管诱导作用;光镜下移植瘤具有与人肾癌类似的组织结构。结论该模型容易建立,能动态观察肾癌诱导血管生成的全过程,可用于肾癌的实验研究。

Objective To establish a human renal cell carcinoma(RCC) model on chick embryo chorioallantoic membrane and to study its biological characteristics.Methods Human RCC cell line RLC-310 was implanted on the chick embryo chorioallantoic membrane.The factors which affect the implanted tumors and the morphological and biological characteristics of the tumors were studied.Results Human RCC model was successfully established.The implanted tumors grew easily on the chick embryo chorioallantoic membrane and had a strong trend of angiogenesis and a structure similar to that of human RCC with microscopic verification.Conclusion This model can be easily duplicated and used in experiment research on human renal cell carcinoma.