一种真菌漆酶的克隆与序列分析

Cloning and Sequencing Analysis of a Laccase Gene from Fungus

比较了几种不同真菌DNA提取方法对血红密孔菌基因组的提取的影响,根据GenBank中真菌漆酶氨基酸保守序列设计的简并引物,以血红密孔菌基因组总DNA为模板,扩增到一条长度为1084bp的DNA片段。通过BLAST比对,其基因序列与密孔菌属同源性最高,为99%,氨基酸同源性为88%,说明扩增的片段确为密孔菌漆酶基因。

Effect of several different methods on the DNA extraction from Pycnoporus sanguineus were compared in this study.Degenerate primers were designed according to the fungal laccase condervation sequences which was registered on GenBank,.A 1084bp laccase gene fragment was amplified by using genomic DNA from Pycnoporus sanguineus as template.According to the BLAST results,we found that the sequence presented a very high match with the laccase gene from Pycnoporus and shared considerable homology of above 99%.The alignment of amino acids shared considerable homology of above 88%.Therefore,the genomic DNA should be Pycnoporus.