生物电化学法转化甘油生产1,3-二羟基丙酮

Bio-Electrochemical Synthesis of 1,3-Dihydroxyacetone from Glycerol

氧化葡萄糖酸杆菌中依赖辅基吡咯喹啉醌(Pyrroloquinoline Quinone,PQQ)的膜结合甘油脱氢酶(Glycerol dehydrogenase,GDH)是酶法转化甘油生成1,3-二羟基丙酮(1,3-Dihydroxyacetone,DHA)的关键酶。以铁氰化钾为电子媒介体,采用生物电化学法,再生甘油脱氢酶的辅基PQQ,从而实现酶法循环转化甘油生产DHA。设计电耦联反应装置,在(28±2)℃,370mV电压下反应18h,DHA质量浓度达到27.21g/L,甘油转化率为52.93%。

The membrane-bound glycerol dehydrogenase(GDH) was the key enzyme of bioconversion of glycerol to 1,3-dihydroxyacetone by Gluconobacter oxydans.The membrane fraction of Gluconobacter oxydans was collected by ultra-centrifugation,and GDH was isolated from the membrane fraction and purified by CM-cellulose column and Sephacryl HR 400 column chromatography.The electrochemical regeneration of the membrane-bound glycerol dehydrogenase was studied by cyclic voltammetry.Using this system,the bio-electrochemical preparation of 1,3-dihydroxyacetone(DHA) from glycerol without coenzyme was investigated.When the temperature was 28±2℃,during 18 h,the DHA production reached at 27.21g/L and the conversion rate of glycerol was 52.93%.