摘要
This paper studied the expert system of genotype discrimination for the STR locus D5S818 based on near-infrared spectroscopy-principal discriminant variate (PDV).Six genotypes,i.e.genotypes 10-10,10-11,11-11,11-12,11-13 and 13-13,were selected as research subjects.Based on the optimum polymerase chain reaction (PCR) conditions,about 54 measuring samples for each genotype were obtained;these samples were tested by near-infrared spectroscopy directly.With differences between homozygote genotypes and heterozygote ones,and differences of the total number of core repeat units between the six genotypes,two types of genotyping-tree structure were constructed and their respective PDV models were studied using the near-infrared spectra of the samples as recognition variables.Finally,based on the classification ability of these two genotyping-tree structures,an optimum expert system of genotype discrimination was built using the PDV models.The result demonstrated that the built expert system had good discriminability and robustness;without any preprocessing for PCR products,the six genotypes studied could be discriminated rapidly and correctly.It provided a methodological support for establishing an expert system of genotype discrimination for all genotypes of locus D5S818 and other STR loci.
This paper studied the expert system of genotype discrimination for the STR locus D5S818 based on near-infrared spectroscopy-principal discriminant variate (PDV).Six genotypes,i.e.genotypes 10-10,10-11,11-11,11-12,11-13 and 13-13,were selected as research subjects.Based on the optimum polymerase chain reaction (PCR) conditions,about 54 measuring samples for each genotype were obtained;these samples were tested by near-infrared spectroscopy directly.With differences between homozygote genotypes and heterozygote ones,and differences of the total number of core repeat units between the six genotypes,two types of genotyping-tree structure were constructed and their respective PDV models were studied using the near-infrared spectra of the samples as recognition variables.Finally,based on the classification ability of these two genotyping-tree structures,an optimum expert system of genotype discrimination was built using the PDV models.The result demonstrated that the built expert system had good discriminability and robustness;without any preprocessing for PCR products,the six genotypes studied could be discriminated rapidly and correctly.It provided a methodological support for establishing an expert system of genotype discrimination for all genotypes of locus D5S818 and other STR loci.
作者
Zai-Zhen Wu a,Jian-Hua Tang a,Bin Zhang a,Li-Ping Guo b,Hong-Ping Xie a,Bing-Ren Gu c a College of Pharmaceutical Sciences,Soochow University,Suzhou 215123,China b College of Chemistry and Chemical Engineering,Chongqing University of Science and Technology,Chongqing 401331,China c Suzhou Institute for Drug Control,Suzhou 215002,China
基金
supported by grants from the National Natural Science Foundation of China (Grant no. 81001686)
