摘要
Objective To survey changes and the significance of phospholipase A_2(PLA_2) on brain tissue of SD rat in acute pancreatitis.Methods With retrograde injection of 3% taurocholate sodium into pancreatic and biliary duct,rat model of severe acute pancreatitis(SAP) was made,and it included four groups: the control group,the sham-operation group, the SAP group and the PLA_2 inhibitor-treated group of SAP.Serum amylases,PLA_2 and PLA_2 in brain tissue were measured and the brain tissue changes were observed.Results There were no significant difference in serum amylases, PLA_2 and PLA_2 in brain tissue between the sham-operation and the control groups;the levels of serum amylases,PLA_2 and PLA_2 in brain tissue in the SAP group were higher than those in the control.In the SAP group expansion and hemorrhage of meninges,intracephalic arteriolar hyperemia,in meninges and cephalic-parenchyma infiltration of inflammatory cells and interval broaden were observed,significant differences were found between two groups.Compared with the SAP group,the level of serum amylase,PLA_2 and PLA_2 in brain tissue were reduced significantly in the treatment group of SAP.Pathological damages in the treatment group were significantly reduced when compared with the SAP group.Conclusion PLA_2 might play an important role in brain tissue damages in severe acute pancreatitis.
Objective To survey changes and the significance of phospholipase A_2(PLA_2) on brain tissue of SD rat in acute pancreatitis.Methods With retrograde injection of 3% taurocholate sodium into pancreatic and biliary duct,rat model of severe acute pancreatitis(SAP) was made,and it included four groups: the control group,the sham-operation group, the SAP group and the PLA_2 inhibitor-treated group of SAP.Serum amylases,PLA_2 and PLA_2 in brain tissue were measured and the brain tissue changes were observed.Results There were no significant difference in serum amylases, PLA_2 and PLA_2 in brain tissue between the sham-operation and the control groups;the levels of serum amylases,PLA_2 and PLA_2 in brain tissue in the SAP group were higher than those in the control.In the SAP group expansion and hemorrhage of meninges,intracephalic arteriolar hyperemia,in meninges and cephalic-parenchyma infiltration of inflammatory cells and interval broaden were observed,significant differences were found between two groups.Compared with the SAP group,the level of serum amylase,PLA_2 and PLA_2 in brain tissue were reduced significantly in the treatment group of SAP.Pathological damages in the treatment group were significantly reduced when compared with the SAP group.Conclusion PLA_2 might play an important role in brain tissue damages in severe acute pancreatitis.
