期刊文献+

The quantitative analysis of S100 in the brain tissue and serum following diffusebrain injury in rats

The quantitative analysis of S100 in the brain tissue and serum following diffusebrain injury in rats
下载PDF
导出
摘要 Objective To investigate the dynamics of the level of S100 in cerebrum,brainstem,and serum following the diffuse brain injury in rats and provide the experimental evidences for estimating injury time.Methods ELISA was used to determine whether S100 protein is changed after diffuse brain injury in rats.Forty rats were sacrificed at 0.5 hour, 2 hours,4 hours,12 hours,24 hours,3d and 7d after diffuse brain injury and normal rats as control.Results The level of S100 in cerebrum,brainstem,and serum increased,followed by a decrease,and then further increased.The level of S100 could be detected to increase at 30 minutes and reached the peak at 4 hours after DBI.The level decreased gradually to the normal at 1d and till 3d formed the second peak.The level returned to the normal at 7d following injury again.In the postmortem injury groups,there were no significant changes compared to the control group.Conclusion The present study showed that the time-dependent expression of S100 is obvious following diffuse brain injury in rats and suggested that S100 will be a suitable marker for diffuse brain injury age determination. Objective To investigate the dynamics of the level of S100 in cerebrum,brainstem,and serum following the diffuse brain injury in rats and provide the experimental evidences for estimating injury time.Methods ELISA was used to determine whether S100 protein is changed after diffuse brain injury in rats.Forty rats were sacrificed at 0.5 hour, 2 hours,4 hours,12 hours,24 hours,3d and 7d after diffuse brain injury and normal rats as control.Results The level of S100 in cerebrum,brainstem,and serum increased,followed by a decrease,and then further increased.The level of S100 could be detected to increase at 30 minutes and reached the peak at 4 hours after DBI.The level decreased gradually to the normal at 1d and till 3d formed the second peak.The level returned to the normal at 7d following injury again.In the postmortem injury groups,there were no significant changes compared to the control group.Conclusion The present study showed that the time-dependent expression of S100 is obvious following diffuse brain injury in rats and suggested that S100 will be a suitable marker for diffuse brain injury age determination.
出处 《Journal of Pharmaceutical Analysis》 SCIE CAS 2007年第1期118-121,共4页 药物分析学报(英文版)
基金 This work was supported by the Doctoral Foundation of Xi an Jiaotong University(No.160-071206)
关键词 S100 diffuse brain injury ELISA S100 diffuse brain injury ELISA
  • 相关文献

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部