摘要
Objective To disclose the mechanism of green tea polyphenol in the prevention ol dental caries.ththods Using sodium fluoride (highly effective anticaries agent) as control and Streptococcus mutans(carigenous microbe) as target, green tea polyphenol (potential bacterial inhibitor) was used to testify its action onthe change of microbial morphology, extramicrobial sucrase, glucosyltranslerase (GTF), lactate hydrogenase (LDH),protein and pH values of the culture media. Results GTF activities were inhibited both by green tea polyphenoland sodium fluoride, the inhibitory ellcacy was stronger in green tea polyphenol. Morphologic changes consisted ofmembrane disroption in tea polyphenol treated microbes, and cytoplasmic vacuolization in fluoride treated bacteria.High protein level in fluoride treated culture media means cytoplasmic decomposition, intracellular protein leakageinto media. Conclusion The inhibition of GTF activity was stronger in green tea polyphenol treated microbes.The target of action was cell membrane disruption in green tea polyphenol treated cell and cytoplasmic in fluoridetreated microbes.
Objective To disclose the mechanism of green tea polyphenol in the prevention ol dental caries.ththods Using sodium fluoride (highly effective anticaries agent) as control and Streptococcus mutans(carigenous microbe) as target, green tea polyphenol (potential bacterial inhibitor) was used to testify its action onthe change of microbial morphology, extramicrobial sucrase, glucosyltranslerase (GTF), lactate hydrogenase (LDH),protein and pH values of the culture media. Results GTF activities were inhibited both by green tea polyphenoland sodium fluoride, the inhibitory ellcacy was stronger in green tea polyphenol. Morphologic changes consisted ofmembrane disroption in tea polyphenol treated microbes, and cytoplasmic vacuolization in fluoride treated bacteria.High protein level in fluoride treated culture media means cytoplasmic decomposition, intracellular protein leakageinto media. Conclusion The inhibition of GTF activity was stronger in green tea polyphenol treated microbes.The target of action was cell membrane disruption in green tea polyphenol treated cell and cytoplasmic in fluoridetreated microbes.
