摘要
Objective To study the effects of wrap-restraint stress and of CRF or CRF antagonist (a-belieal-CRF9-41 ) microinjection on gastric emptying, and the role of plasma motilin (Mot ). Methods The effects of wrap- restraint stress or CRF and CRF antagonist microinjection into paraventricular nucleus (PVN) on gastric empiring were determined by using dextran blue-2000 as a marker. The plasma level of Mot was determined by radioimmunoassay. Results Wrap-restraint stress inhibited gastric emptying. Microinjection of CRF into PVN inhibited gastric emptying dose-dependently. In normal condition, a-he- lical-CRF9-4l bilaterally microinjected into PVN didn’ t modify gastric emptying; however, a-belieal-CRF9-41 microinjection bilaterally into PVN 15min before wrap-restraint stress could significantly antagonize the effect of stress on gastric emptying. After 30min of stress or CRF microinjection into PVN, plasma levels of Mot significantly decreased, as compered with the normal control group. Conclusion Wrap-restraint stress can inhibit gastric emptying, and microinjection of CRF into PVN has the similar effect. The PVN is one of the speific sites for the action of CRF affecting on gastric emptying. Plasma Mot might partic ipate in the effects of stress and CRF-induced alteration of gastric emptying.
Objective To study the effects of wrap-restraint stress and of CRF or CRF antagonist (a-belieal-CRF9-41 ) microinjection on gastric emptying, and the role of plasma motilin (Mot ). Methods The effects of wrap- restraint stress or CRF and CRF antagonist microinjection into paraventricular nucleus (PVN) on gastric empiring were determined by using dextran blue-2000 as a marker. The plasma level of Mot was determined by radioimmunoassay. Results Wrap-restraint stress inhibited gastric emptying. Microinjection of CRF into PVN inhibited gastric emptying dose-dependently. In normal condition, a-he- lical-CRF9-4l bilaterally microinjected into PVN didn' t modify gastric emptying; however, a-belieal-CRF9-41 microinjection bilaterally into PVN 15min before wrap-restraint stress could significantly antagonize the effect of stress on gastric emptying. After 30min of stress or CRF microinjection into PVN, plasma levels of Mot significantly decreased, as compered with the normal control group. Conclusion Wrap-restraint stress can inhibit gastric emptying, and microinjection of CRF into PVN has the similar effect. The PVN is one of the speific sites for the action of CRF affecting on gastric emptying. Plasma Mot might partic ipate in the effects of stress and CRF-induced alteration of gastric emptying.
