一个有Ds插入引起的泡状细胞异常水稻卷叶突变体

A Ds-tagged rice rolling leaf mutant abnormal in bulliform cells

基于Ac/Ds插入突变系统,筛选到一株水稻卷叶突变体.对该突变体进行了初步的表型观察和叶片组织的解剖结构显微观察.利用PCR技术对Ds插入进行了验证,同时,扩增分离了Ds侧翼序列,通过在线比对分析了Ds在该突变体基因组上的插入位点.结果显示,该突变体在幼苗期和成熟期均呈稳定的卷叶表型,叶片泡状细胞在数目和形状上存在明显不同于野生型的异常.研究还证实,突变体基因组含Ac/Ds双元件,Ds定位于水稻第10号染色体上.因而,该突变体为Ds插入的泡状细胞异常的水稻卷叶突变体.本研究为在分子水平上揭示Ds标记基因在泡状细胞发育和分化中的作用奠定了基础.

A rolling leaf rice mutant was obtained by Ac/Ds transposon mutagenesis methods.In this research,we examined the phenotypes of this mutant,and visualized the anatomical structures in leaf tissues.The Ds insertion was also determined by PCR amplification,and the specific sequence flanking the Ds element was isolated by TAIL-PCR technique and then further sequenced.The site of Ds insertion was located by online BLAST sequence alignment algorithms.The results showed that the mutant exhibited a stable rolling leaf phenotype at both seedling and maturing stages,and the bulliform cells appeared abnormal in both number and shape,making them quite different from the fan-shaped structures found in wild-type leaves.Our results also indicated that the mutant genome harbored two components of Ac/Ds system,and the Ds element was identified to locate on chromosome 10.Therefore,it is suggested that this mutant is a kind of Ds-tagged rice rolling leaf mutant abnormal in bulliform cells.These results will provide a fundamental clue to define the roles of Ds-tagged gene in bulliform cell development and differentiation at the molecular level.